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Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro
2016
Das, Krishna | Dupont, Aurélie | De Pauw-Gillet, Marie-Claire | Debier, Cathy | Siebert, Ursula | MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
peer reviewed | Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 µM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 µM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential. To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea.
Show more [+] Less [-]Microplastics in oceans
2011
Zarfl, C. | Fleet, D. | Fries, E. | Galgani, F. | Gerdts, Gunnar | Hanke, G. | Matthies, M.
Environmental and welfare friendly housing for pigs
2010
Aarnink, A.J.A. | Groenestein, C.M. | Ogink, N.W.M.
Thermodynamics and kinetic processes at coal interface for CO2 geological storage
2008
Charriere, Delphine | Pokryszka, Zbigniew | Behra, Philippe
Currently, the geological storage of CO2 is heavily studied around the world in order to limit global warming due to the greenhouse effect. The atmospheric CO2, which has been steadily increasing for more than a century, is assumed to be in a large part responsible of this warming. Nowadays, various options have been considering to store CO2 in an underground environment for periods covering several centuries. Among the options, one is to inject it in unexploited coal seams. The objective of our work is to characterise the interactions between CO2 ans coal interfaces from two French coal seams. This will contribute to identify the most adapted coal basin which could be used for storing CO2 in France.
Show more [+] Less [-]Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdansk (southern Baltic).
2006
Kopecka, J. | Lehtonen, K. K. | Barsiene, J. | Broeg, Katja | Vuorinen, P. J. | Gercken, J. | Balk, L. | Pempkowiak, J.
Determination of organohalogenated contaminants in liver of harbour porpoises (Phocoena phocoena) stranded on the Belgian North Sea coast
2002
Covaci, Adrian | Van de Vijver, Kristin Inneke | De Coen, Wim | Das, Krishna | Bouquegneau, Jean-Marie | Blust, Ronny | Schepens, Paul | MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
peer reviewed
Show more [+] Less [-]Ecologie des principaux macrophytes aquatiques du scorff, application à la bioindication
1995
Daniel, H. | Haury, J.
Prioritization based on risk assessment to study the bioconcentration and biotransformation of pharmaceuticals in glass eels (Anguilla anguilla) from the Adour estuary (Basque Country, France)
2022
Alvarez-Mora, Iker | Bolliet, Valérie | Lopez-Herguedas, Naroa | Castro, Lyen | Anakabe, Eneritz | Monperrus, Mathilde | Etxebarria, Nestor | Department of Analytical Chemistry, University of the Basque Country UPV/ EHU ; Universidad del País Vasco [Espainia] / Euskal Herriko Unibertsitatea [España] = University of the Basque Country [Spain] = Université du pays basque [Espagne] (UPV / EHU) | Plentzia Marine Station, University of the Basque Country ; Universidad del País Vasco [Espainia] / Euskal Herriko Unibertsitatea [España] = University of the Basque Country [Spain] = Université du pays basque [Espagne] (UPV / EHU) | Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP) ; Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Department of Organic and Inorganic Chemistry, University of the Basque Country | Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) ; Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | Agencia Estatal de Investigaci ́on (AEI) of Spain | European Regional Development Fund through project CTM2017–84763-C3–1-R | Basque Government through the financial support as a consolidated group of the Basque Research System (IT1213–19) | University of the Basque Country | Université de Pau et des Pays de l'Adour
International audience | The presence of contaminants of emerging concern in the aquatic environment directly impacts water-living organisms and can alter their living functions. These compounds are often metabolized and excreted, but they can also be accumulated and spread through the food chain. The metabolized contaminants can also lead to the formation of new compounds with unknown toxicity and bioaccumulation potential. In this work, we have studied the occurrence, bioconcentration, and biotransformation of CECs in glass eels (Anguilla anguilla) using UHPLC-HRMS. To select the target CECs, we first carried out an environmental risk assessment of the WWTP effluent that releases directly into the Adour estuary (Bayonne, Basque Country, France). The risk quotients of every detected contaminant were calculated and three ecotoxicologically relevant contaminants were chosen to perform the exposure experiment: propranolol, diazepam, and irbesartan. An experiment of 14 days consisting of 7 days of exposure and 7 days of depuration was carried out to measure the bioconcentration of the chosen compounds. The quantitative results of the concentrations in glass eel showed that diazepam and irbesartan reached BCF ≈10 on day 7, but both compounds were eliminated after 7 days of depuration. On the other hand, propranolol's concentration remains constant all along with the experiment, and its presence can be detected even in the non-exposed control group, which might suggest environmental contamination. Two additional suspect screening strategies were used to identify metabolization products of the target compounds and other xenobiotics already present in wild glass eels. Only one metabolite was identified, nordiazepam, a well-known diazepam metabolite, probably due to the low metabolic rate of glass eels at this stage. The xenobiotic screening confirmed the presence of more xenobiotics in wild glass eels, prominent among them, the pharmaceuticals exemestane, primidone, iloprost, and norethandrolone. ☆ This paper has been recommended for acceptance by. Eddy Y. Zeng. ☆☆ Contaminants of Emerging Concern in Glass Eel (Anguilla anguilla): Occurrence, Bioconcentration and Biotransformation.
Show more [+] Less [-]Modelling bioaccumulation of heavy metals in soil-crop ecosystems and identifying its controlling factors using machine learning
2020
Hu, Bifeng | Xue, Jie | Zhou, Yin | Shao, Shuai | Fu, Zhiyi | Li, Yan | Chen, Songchao | Qi, Lin | Shi, Zhou | Unité de Science du Sol (Orléans) (URSols) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences ; Zhejiang University [Hangzhou, China] | Institute of Land Science and Property, School of Public Affairs ; Zhejiang University [Hangzhou, China] | School of Earth Sciences [Hangzhou] ; Zhejiang University [Hangzhou, China] | InfoSol (InfoSol) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Ningbo Planting Management Station ; Ningbo University (NBU)
The prediction and identification of the factors controlling heavy metal transfer in soil-crop ecosystems are of critical importance. In this study, random forest (RF), gradient boosted machine (GBM), and generalised linear (GLM) models were compared after being used to model and identify prior factors that affect the transfer of heavy metals (HMs) in soil-crop systems in the Yangtze River Delta, China, based on 13 covariates with 1822 pairs of soil-crop samples. The mean bioaccumulation factors (BAFs) for all crops followed the order Cd > Zn > As > Cu > Ni > Hg > Cr > Pb. The RF model showed the best prediction ability for the BAFs of HMs in soil-crop ecosystems, followed by GBM and GLM. The R2 values of the RF models for the BAFs of Zn, Cu, Cr, Ni, Hg, Cd, As, and Pb were 0.84, 0.66, 0.59, 0.58, 0.58, 0.51, 0.30, and 0.17, respectively. The primary controlling factor in soil-to-crop transfer of all HMs under study was plant type, followed by soil heavy metal content and soil organic materials. The model used herein could be used to assist the prediction of heavy metal contents in crops based on heavy metal contents in soil and other covariates, and can significantly reduce the cost, labour, and time requirements involved with laboratory analysis. It can also be used to quantify the importance of variables and identify potential control factors in heavy metal bioaccumulation in soil-crop ecosystems.
Show more [+] Less [-]DNA damage in caged Gammarus fossarum amphipods: A tool for freshwater genotoxicity assessment | Dommages à l'ADN chez l'amphipode Gammarus fossarum encagés : un outil pour le suivi de la génotoxicité des milieux dulçaquicoles
2011
Lacaze, Emilie | Devaux, Alain | Mons, R. | Bony, Sylvie | Garric, Jeanne | Geffard, Adeline | Geffard, Olivier | Milieux aquatiques, écologie et pollutions (UR MALY) ; Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF) | Institut National de la Recherche Agronomique (INRA) | Université de Reims Champagne-Ardenne (URCA)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA | International audience | The aim of this study was to propose a tool for freshwater environmental genotoxicity assessment using Gammarus fossarum, a high ecologically relevant species. In a first part, gammarids were caged upstream and downstream wastewater treatment plant effluent output. The sensitivity of genotoxic responses of haemocytes, oocytes and spermatozoa was compared using the Comet assay. Spermatozoa appeared to be the most sensitive, suitable and relevant cell type for genotoxicity risk assessment. In a second part, a watershed-scale study was conducted over 2 years to evaluate the applicability of our caging procedure. The genotoxic impact of a contamination was followed, taking into account seasonal variability. DNA damage in spermatozoa exhibited low basal level and low variability in control upstream sites, providing a reliable discrimination of polluted sites. Finally, DNA damage in caged G. fossarum has been proved to be a sensitive and reproducible tool for freshwater genotoxicity assessment.
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