The production of innovative composition (high omega group fatty acids and antioxidants-carotenoids) broiler chicken meat and egg opportunities in Latvia was assessed. After feeding the poultry with feed enriched with fatty acids and antioxidants, broiler meat and eggs of innovative composition containing a higher amount of omega-3 (in meat by 1.7%, in eggs by 2.7%), omega-6 (in meat by 3.9%, in eggs by 3.2%) and carotenoids (0.44 mg kg-1 in meat and in eggs by 6.9 mg kg-1) when compared with the content of commercial products are obtained. Nutrition costs of innovative composition-based broiler chicken meat production are LVL 20 higher and nutrition costs of egg production are LVL 1.49 higher per 1000 units of output than in the standard version of the poultry feeding-stuff. The poultry farming production of an innovative composition, however, is economically profitable due to the higher rates of poultry productivity. In the case of an innovative composition-based yield the potential profit at the currently equal cost realisation of poultry meat and eggs is higher when calculating 293.47 LVL per 1000 broiler chickens and 5.52 LVL per 1000 eggs in comparison with commercial production.

There are different methods of egg yolk oil extraction, but still solvent extraction is commonly used. Due to the high cost of egg yolk powder production, extraction of lipids from liquid egg yolk remains very topical. Crude egg yolk oil obtained by solvent extraction from liquid egg yolk contains high amount of water which can decrease shelf life of egg oil promoting lipid oxidation. High concentration of residual solvents limits the usage of egg oil in food or in cosmetics due to the health risks. The aim of this study is to purify egg yolk oil obtained by solvent extraction from liquid egg yolk. Results show that it is possible to decrease the water content in egg oil from 14.26 ± 1.29% to 0.88 ± 0.13% by eliminating lecithin from egg oil. Solvent evaporation in the rotary film evaporator under the vacuum cannot remove solvents completely from the extract, but nitrogen streaming through the extract as a last step in evaporation process removes solvent residues, leaving behind high quality egg yolk oil suitable for food application.

This article presents the results of studies of the chemical composition of the fish meat while using as a feed additive zeolite of Chankanay origin. The research was conducted during 2011-2013 at the Department of Veterinary-sanitary examination and hygiene of the Kazakh National Agrarian University and at the laboratory of JSC ‘Kazakh Academy of Nutrition’ LTD ‘NUTRITEST’. The material of study was the zeolitic tuff of Chankanay deposit (Almaty region) and two-year trout Oncorhynchus mykiss. For the research we used feed by recipes of GosNIORH - 12-80. The chemical composition of fish meat was determined by a set of methods: moisture by drying at 105 °C, fats by Soxhlet, total protein by modified Kjeldahl method (ISO 937:1974), and minerals by incineration in a muffle furnace. Calorie content of meat was determined by Alexandrov’s formula: X = C - (F+A) ´ 4.1 + F ´ 9.3. Fatty acid composition of fish meat was determined by gas-liquid chromatography. Thus, the results are the indirect evidence that zeolites added to primary diet for growing fish, do not adversely impact proteolytic enzyme systems of fish, that is, have no negative effect on fish organism.

Every autumn and winter period, game animals - elk (Alces alces), deer (Cervus elaphus), roe deer (Capreolus capreolus) and wild boar (Sus scrofa scrofa) - provide an excellent input in the diversification of many consumer meals. In the recent years, consumption and assortment of game meat products has significantly increased. At the moment there are only few biochemical composition investigations of the game meat. The meat of wild animals is more favourable for human health, because it has a lower content of saturated fatty acid, but a higher content of protein. Therefore the aim of our investigation was to compare biochemical composition of the game meats hunted in Latvia. Investigations were carried out in different regions of Latvia. In the studied samples, protein, intramuscular fat, fatty acids and cholesterol were determined. The biochemical analysis of 54 samples was carried out. The results of the analysis showed that protein content in all types of the meat samples was 22.36 - 22.92%, which is not statistically significant. The fat content, on the other hand, was significantly lower: 1.33 ± 0.88% in elk meat samples, and 1.59 ± 0.59% in roe deer meat samples. Content of cholesterol varied from 64.41% to 95.07% in the ruminant meat samples of different species. From the dietetic point of view, the meat samples of roe deer had the best composition of fatty acids.

Nutritional value and shelf life of milk and dairy products depend on the composition and stability of their constituents. The aim of the present study was to evaluate the effects of carrots as cow feed carotenoid source on butter oil fatty acid (FA) composition and oxidative stability. Milk was obtained from one trial group (TG; n=5) and one control cow group (CG; n=5) in a conventional dairy farm in Latvia. TG cows received carrots 7 kg per cow per day; the length of the supplementation period was 39 days. The stability of butter oil exposed to sunlight (3h) and held for 14 days in the temperature of 60 °C was analyzed by peroxide value method. The changes of the FA content and ratios in TG milk fat were more positive with respect to the fat nutritional value as observed in CG – stronger increase in the content of polyunsaturated FA (p is less than 0.05), and in the ratio between stearic and palmitic acids (p is less than 0.05); also a tendency was seen of increasing content of short and monounsaturated FA, as well as decreasing the ratio between ω6 and ω3 FA groups. Oxidative stability of the carrot supplemented cow group’s milk butter oil samples that were collected after 25- day trial period, exposed to sunlight (3h) and stored at a temperature of 60 °C was significantly (p is less than 0.05) higher compared to CG samples. After the 39-day long trial period stability difference was not significant, despite the tendency that average polyunsaturated FA content in TG samples was higher compared to the control.

The purpose of this study was to evaluate the effect of probiotics, herbals and buckwheat bran (Fagopyrum esculentum L.) on growth, profile of blood, gut microbiota, profile of fatty acid in meat and shedding of resistant Escherichia coli (E. coli) in piglets. A total of 44 piglets (Sus scrofa domesticus) from age of day 14 to 56 were divided into 4 groups. Control received basal diet (group C), basal diet + probiotics (group P), basal diet + 3% buckwheat bran (group PB) and basal diet + 1.5% herbals (group H). No effect was observed in growth in all groups. The count of Lactobacillus spp. increased (p is less than 0.05) in jejunum in group P. In the faeces, Enterobacteriaceae decreased in the group P (p is less than 0.05) of 35 days old piglets, but Enterobacteriaceae and E. coli decreased in the group H (p is less than 0.05) of 56 days old piglets. The prevalence of resistance to at least one antibiotic class was 66.7% before and 50% after the experiment in all groups. Multidrug resistance of E. coli was not observed in 14 days old piglets, but was observed in 50% and more in all of study groups of 56 days old piglets. The fatty acid composition of Longissimus thoracis muscle had higher levels of α-linolenic acid and palmitoleic acid (p is less than 0.05), but lower level of stearic acid (p is less than 0.05) in group P. In conclusion, probiotics and herbals improved gut microbiota, fatty acid profile and affected shedding of resistant E. coli, but not growth performance.

Vegetable oils are essential providers of energy to a human body; they play important role as a foodstuff. During processing and storage of vegetable oils, they may undergo molecular changes adverse to the human organism affecting the quality of the oils. The content of fatty acids of fresh rapeseed, linseed and hemp oils and of the mixture of rapeseed (800 g kgE-1) and linseed (200 g kgE-1) oils was determined by the method of gas chromatography (GH). Alpha - , delta - and gamma - tocopherols were analyzed in the vegetable oils by the methods of the highly effective liquid chromatography (HPLC). The hemp oils contain the indispensable polyunsaturated fat acids: linoleic acid - 53.0%, and linolenic acid - 23.2%. Such proportion of the fatty acids (3:1) is considered optimal in the nutrition. When using both hot and cold pressure technology for the obtaining of oil, it is possible that the polycyclical aromatic hydrocarbon - Benzo[a]pyrene - may be created and influenced the quality of the oil. The environment also affects the content of the lead in the oils. The density of the various vegetable oils at the temperature of 20 +- deg C proved to be within the limits of 0.917 and 0.942 kg dmE-3.

This research presents the antiradical activity and fatty acids changes of vegetable oils. Natural antiradical activity and its changes during storage for one and two years of rapeseed, linseed and hemp oils were determined with 1.1-diphenyl-2-picrylhydrazyl (DPPH). Fresh hemp oil shows the higher antiradical activity. The content of fatty acids of fresh rapeseed, linseed and hemp oils and of the mixture of rapeseed (800 g kgE-1) and linseed (200 g kgE-1) oils was determined by the method of gas chromatography. After heating the mixture of rapeseed and linseed oils at the temperature of 160-180 deg C, changes proportions in saturated and unsaturated fatty acids.

The aim of the present study was to determine the level of cholesterol in the milk of the Latvia's cow herds, analyse differences between two different methods of cholesterol determination in milk. Milk samples were obtained from the cows of Latvian Brown and Black and White breeds. The milk fat and protein were analysed by Milkoscan 133, cholesterol content was determined colorimetricaly by Blur procedure with prior lipid extraction by Roese-Gottlieb method and by gas-chromatography method. The obtained results showed that the milk fat content was 4.48+-0.28%, protein was 3.03+-0.09%, cholesterol content in milk was 21.05+-2.01 mg 100 ml*[-1), when assayed by spectrophotometer SF 26 LOMO and 18.29+-1.73 mg 100 ml*[-1), when assayed by gas chromatography. There were no significant differences in cholesterol content between both determination methods.

The goal of this study was to investigate the use of body reserves, energy balance and fertility parameters at the beginning of lactation in first-parity cows bred in Estonia.