The study was aimed to investigate the effect of potato (Solanum tuberosum L.) in vitro plantlets planting density under greenhouse conditions on obtained minitubers number per unit area, multiplication rate and their weight distribution. Three cultivars of different maturity (‘Monta’ – early maturity, ‘Prelma’ – medium early and ‘Mandaga’ – medium late maturity) were used for the study. In vitro plantlets were planted in a greenhouse of State Priekuli Plant Breeding Institute, Latvia in 2014 at four planting densities (PDs) 63 plants per square m, 95 plants per square m, 142 plants per m2 and 184 plants per square m respectively. Fertilized peat was used as a substratum. Significant effect of planting density (p is less than 0.001) and cultivar (p is less than 0.01) was found on analyzed yield parameters. Increased planting densities resulted in increased minitubers number per square m (from 272 minutubers mE-2 at PD 63 plants mE-2 to 414 minutubers mE-2 at PD 184 plants mE-2), decreased multiplication rate (4.3 to 2.7 minitubers per planted plant) and mean fresh weight of minitubers (from 20.26. g to 12.11 g). The highest increase of minitubers number per m2 was observed within size (weight) range 3 to 5 g. Minitubers number per m2 increase within bigger size ranges (5 to 10 g, 10 to 20 g) was less pronounced. Slight insignificant (p=0.330) decrease of minitubers number is greater than 20 g was observed in relation of planting density increase (112 minitubers mE-2 at PD 95 plants mE-2 to 84 minitubers mE-2 at PD 184 plants mE-2).

Light is one of the most significant environmental factors affecting the growth of plants, as it is the driving force of photosynthesis. Among others, the red and blue light are the most relevant, as these spectral regions are absorbed by chlorophyll the most. In addition, red and blue light trigger specific photomorphogenic responses that allow plants to capture the available light efficiently. Accordingly, the proportion of red and blue light (R:B ratio) is considered one of the most important characteristics of light for plants, as optimal R:B provides balanced growth and photosynthesis. The aim of this study was to evaluate how the in vitro cultures of hybrid aspen (Populus tremula L. × P. tremuloides Michx.) are affected by different R:B ratios under fixed illumination intensity. We examined the growth characteristics of plantlets under wide spectrum LED luminaires with three different R:B proportions – 1:1, 7:3 and 9:1. While photosynthesis-related variables were significantly affected by light, the effect on morphology was less pronounced. Overall, increased proportions of red light decreased the photosynthetic performance of plantlets without giving significant benefits in the form of longer shoots that could be used to facilitate propagation effectiveness. Nonetheless, the effect of light treatment remains at least partially clones-specific and should be considered in case of further application for propagation purposes.