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Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes
2015
Celal Türker | Bahri Devrim Özcan
In the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for CT1 strain, and at 80°C for CT2 and CT3 strains, respectively. The optimum enzyme activity was observed at 90°C for CT1 α-amylase, whereas at 60°C for CT2 and CT3 α-amylases. On the other hand, optimum pH value for CT2 α-amylase was 7.0, whereas 8.0 for CT1 and CT3 α-amylases. The specific activities of CT1, CT2, and CT3 amylases were 317.6, 113.3 and 362.7 U/mg at 55°C, respectively. The estimated molecular weight of CT1 and CT3 α-amylase was 65 kDa, and for CT2 α-amylase was 38 kDa by zymogram analysis.
Show more [+] Less [-]The relationships between phenylthiocarbamide taste perception and smoking, work out habits and susceptibility to depression
2015
Sevgi Durna Daştan | Yusuf Muhammed Durna | Taner Daştan
Phenylthiocarbamide (PTC) is known as phenylthiourea and it is an organic compound that has the phenyl ring. Ability to perceive the tastes of PTC chemical is related to the dominance of taste genes. There are a large number of population studies regarding the PTC taste perception and different personal characteristics or disease conditions. The purpose of this study is to reveal and compare the relation between the PTC taste perception and work-out habits, smoking, alcohol consumption and tendency to the depression of people. A total of 2500 adults were volunteered to be included in this study. PTC taste perception was measured by tasting with PTC solution (10 mg/L) filtered in a paper. It showed that tasters were significantly more frequent (81.8%) than nontasters (18.2%) in all population. And in some parameters analyzed in this study, there are significant differences. The taste genetics show up with environmental factors and create the sense of taste, which develops the feeding behaviors. The taste perception resulting from food and beverages diversifies by genetic and environmental effects and the nervous system interprets this perception. This study is enlightening in terms of presenting that the taste perception of people affects their lifestyles and lead them to start and either continue or discontinue some habits.
Show more [+] Less [-]Evaluation of Different Groundnut Arachis hypogea L. Cultivars Against Termites, Odontotermes obesus (Rambur) in Rawalpindi, Pakistan
2015
Muhammad Usman Malik | Humayun Javed | Mahmood Ayyaz
Six groundnut (Arachis hypogea L.) cultivars i.e. Golden, BARI-2000, Australian, BARD-479, ICG-7326 and 2KCGC20 were evaluated against the attack of Odontotermes obesus in terms of percentage infestation of workers and soldiers, their damage and impact on the yield. The maximum mean populations of O. obesus workers were recorded on 2KCGC20 (30.82±2.75 workers/plot) and Australian (30.65±2.92 workers/plot), followed by BARI 2000 (19.55±2.51 workers/plot), Golden (17.30±2.27 workers/plot) and BARD-479 (16.82±2.06 workers/plot). The minimum mean population of O. obesus workers was found on ICG-7326 cultivars (14.85±1.81) workers/plot. The maximum mean population infestation of O. obesus soldiers was recorded on 2KCGC20 cultivar (4.88±0.25 soldiers/plot) followed by Australian (4.15±.30, soldiers/per plot), BARD-479 (2.00±.17 soldiers/plot), ICG-7326 (1.70±.14 soldiers/plot), BARD 2000 (2.40±.25 soldiers/ plot). The minimum mean population of O. obesus soldiers was found on Gold (1.87±.17 soldiers/plot). Population of O. obesus workers was positively related with the damage and negatively related the yield in all the groundnut cultivars.
Show more [+] Less [-]The Aggregation of Boron on the Tissues of Gold Fish (Carassius auratus Linnaeus, 1758)
2015
Tuncer Okan Genç | Burak Evren İnanan | Murat Yabanlı | Fevzi Yılmaz
In this study, it was aimed to determine the water-borne and food-borne boron accumulation in the liver and muscle tissues of Gold Fish (Carassius auratus Linnaeus, 1758). For each treatment, 12 individuals were. The water-borne boron treatments were applied as boron acid concentration of 1 mg/L, 10 mg/L and 20 mg/L in the aquarium water, while the food-borne boron treatments were prepared food contained the defined levels of boron (1 mg/kg, 5 mg/kg and 10 mg/kg) as boric acid. The boron levels in the tissues were determined by an ICP-MS procedure. The maximum boron concentration was found in the 20mg/L water borne boron treatment in the liver tissue (1.78±0.02 mg/kg). In the water-borne boron treatments, the maximum Transfer Factor (TF) was found in the 20mg/L boron concentration, and TF values were increased when the boron concentrations were decreasing. In the 1 mg/kg food-borne boron treatment, TF was found as 0, and increasing concentration of boron in the food caused an increase in TF reached about 0.06. This study suggested that the target organ for boron accumulation is the liver rather than the muscles and the accumulation of food-borne boron is lower when comparing water-borne boron.
Show more [+] Less [-]The Symptoms of Herbicidal Action: The Case of Aclonifen
2015
Özgür Kıvılcım Kılınç
The symptoms which were directly bound to the biochemical mode of action of an herbicide, other symptoms result from an indirect consequence of this action. The symptoms of herbicidal action deeply differed and that the climatic factors during the two first weeks after treatment could change definitely the result of the selective herbicide action. The repetitive observation of symptoms allows to inform the farmer about the tolerance or resistance of certain plant species, including the culture, for instance through the appearance of symptoms on the first leaves of the seedlings and their absence in the following leaves. As a whole, the accurate observation of herbicidal symptoms on plants is the essential, rapid and non-expensive analysis of treatment effectiveness at the field scale. The purpose of the current report is to describe the symptoms of a very complex herbicidal action, that of aclonifen involving two modes of action for the same molecule, approximately at the same concentration, and to compare these symptoms under field conditions and under controlled conditions, for a better understanding.
Show more [+] Less [-]A New Approach to Determine Time and Temperature Combination for Electrical Conductivity Test in Sorghum
2015
Mehmet Demir Kaya | Onur İleri
This study was conducted to determine a suitable time and temperature combination for the electrical conductivity test to be used in sorghum seeds. Fifty seeds known initial seed moisture content and weight of fresh and dead seeds (105°C for 6h) of seven sorghum cultivars were used as material. The electrical conductivities of soaking water were measured using an EC meter in 20, 25 and 30°C for 4, 8, 12 and 24 h using 50 mL deionized water. The experimental design was three factor factorial (7 × 3 × 4) arranged in a completely randomized design; with four replications and 50 seeds per replicate. The results showed that increased time and temperature caused a remarkable increase in EC values of all of the cultivars. Temperature significantly affected the electrical conductivity values and the best results were obtained at 25°C. The cultivars with the lower germination percentage gave the higher electrical conductivity value. Dead seeds always gave higher electrical conductivity at 25°C for all periods. It was concluded that the temperature of 25°C and 24 h was the optimum combination for the electrical conductivity test in sorghum.
Show more [+] Less [-]Antioxidant Activity and Phenolic Content of Apple Cider
2015
Nilgün Havva Budak | Filiz Ozçelik | Zeynep Banu Güzel-Seydim
Fruit and vegetables are an important component of a healthy diet and the main antioxidant suppliers in the human diet. Consumption of foods derived from fruits and vegetables is also essential; fruit juices, ciders, wines, and vinegars also contain significant amounts of polyphenolic compounds. The aim of the study was to determine the effect maceration of antioxidant activity and phenolic content of apple cider. Red delicious apples were used to produce natural apple cider with and without inclusion of maceration. Samples were taken from fresh red apple juice, macerated samples and apple cider. Apple cider (maceration was applied) (CAM) had the highest total phenolic content, chlorogenic acid, ORAC and TEAC levels. Chlorogenic acid was the dominant phenolic substance in apple juice and cider samples and chlorogenic acid was increased with maceration process.
Show more [+] Less [-]Koçansız Şeker Mısırı Silajlarının Kalitesine Sodyum Format Katkısının Etkisi
2023
Asuman Arslan Duru | Behlül Sevim | İlker İnal | Bülent Çakır | Osman Olgun | Tugay Ayaşan
Bu çalışma, farklı düzeylerde organik asit temeline dayalı sodyum format (SF) ilavesinin koçansız şeker mısır silajlarının ham besin madde içerikleri, fermentasyon kalitesi, kuru madde tüketimi, sindirilebilir kuru madde ve nispi yem değeri üzerine etkisini tespit etmek amacıyla yürütülmüştür. Araştırmada, koçansız mısır hasıllarına %0 SF (kontrol grubu); %1 SF ve %2 SF ilave edilerek gruplar oluşturulmuş ve 60 gün fermentasyona bırakılmıştır. Araştırma sonunda, SF katkısının mısır silajlarının kuru madde, ham protein, ham yağ, ADF, NDF, nişasta, ME, asetik asit, propiyonik asit, bütirik asit ve etanol içeriklerini azalttığı, buna karşılık laktik asit, ham kül ve nişasta düzeylerini artırdığı tespit edilmiştir. Ayrıca araştırma silajlarının pH değerlerinin, %1 SF ilavesiyle istatistiksel olarak önemli düzeyde azaldığı belirlenirken; kuru madde tüketimi, sindirilebilir kuru madde ve nispi yem değerlerinin ise arttığı tespit edilmiştir. Araştırma sonunda, %1 SF ilavesinin koçansız mısır silajlarının pH’sını düşürücü ve laktik asit artırıcı etkisinin yanı sıra kuru madde tüketimi, sindirilebilir kuru madde ve nispi yem değerleri üzerine pozitif etkisi nedeniyle kullanılabileceği sonucuna varılmıştır.
Show more [+] Less [-]Evaluation of the Blood Gas Parameters Changes During Ovariohysterectomy with Butorphanol, Medetomidine, and Ketamine Combination Anesthesia in Queens
2023
Fatma Satılmış | Merve İder | Muhammed Furkan Çiftçi | Ömer Faruk Yeşilkaya | Berrak Işık Soytürk | Hasan Alkan | Kübra Karakaş Alkan
Sunulan çalışmanın amacı kedilerde ovaryohisterektomi sırasında butorfanol, medetomidine ve ketamin kombinasyonu ile yapılan anestezinin kan gazı parametrelerine etkisinin belirlenmesidir. Çalışmada 6-72 ay aralığında, farklı ırklarda, 19 adet kısırlaştırılan dişi kedi kullanıldı. Anesteziden önce vena cephalica antebrachi’den heparinli enjektöre 1 mL kan alınarak kan gazı parametreleri değerlendirildi. Klinik muayene ve laboratuvar analizler sonucunda sağlıklı olduğu belirlenen kediler ovaryohisterektomi için genel anesteziye alındı. Anestezi indüksiyonu için butorfanol (0,01 mg/kg, IV) uygulandı, uygulamadan 5 dakika sonra medetomidine (0,08 mL/kg, IV) ve 10 dakika sonrasında ise ketamin (5-7,5 mg/kg, IV) damar içi yolla verildi. Ketamin uygulamasının 10. dakikasında kedilerden tekrar kan örneği alındı. Anestezi öncesi ve sırasında alınan kan örneklerinde pH, pCO2, pO2, sO2, Na, Ca, K, Cl, Glu, Lac, BE ve HCO3 düzeyleri değerlendirildi. Anestezisi indüklenen kedilerde pH, pO2, sO2, BE ve HCO3 düzeylerinin anestezi öncesine göre daha düşük, pCO2 ve laktat konsantrasyonlarının ise yüksek olduğu tespit edildi. Sonuç olarak ovaryohisterektomi uygulanacak kedilerde kan gaz parametreleri takibinin yapılmasının, operasyon öncesi ve sonrası oluşabilecek komplikasyonların önlenebilmesi için yararlı olabileceği düşünüldü.
Show more [+] Less [-]Süt Sığırı Çiftliklerinde İyi Tarım Uygulamaları, Globalgap, AB ve TR Organik Sistemlerin Analizi ve Bunların Hayvan Refahı Standartları Bakımından Karşılaştırılması
2023
Adil Akın Aydın | Ali Şenok | Gürsel Dellal
Çok önemli bir hayvansal protein kaynağı olan süt, dünyada ve Türkiye’de esas olarak sığırlardan elde edilmektedir. Diğer çiftlik hayvanlarında olduğu gibi sığırlarda da iyi bir hayvan refahının sağlanamaması durumunda istenilen verim düzeyine ulaşılamamakta ve önemli ekonomik kayıplar meydana gelmektedir. Son yıllarda süt sığırı çiftliklerinin sürdürülebilir üretim yapabilmesi için sığır refahının iyi bir şekilde yönetilmesine yönelik çalışmalarla birlikte birçok ülkede hayvan refahının daha etkili yönetildiği geleneksel üretim sistemleri dışındaki diğer üretim sistemlerini uygulayan çiftliklerin sayısında da önemli artışlar gerçeklemiştir. Bu üretim sistemlerinden Türkiye’de uygulananlar İTU, GlobalGap, TR-Organik ve AB-Organik sistemleridir ve özellikle Avrupa Yeşil Antlaşması’nın yayınlanmasından ve ülkemizin Paris Antlaşması’na taraf olmasından sonra bu sistemlerin daha da gelişeceği beklenmelidir. Bu noktadan hareketle bu makalede; Türkiye’de İTU, GlobalGap ve organik kapsamda üretim yapan süt sığırcılığının mevcut durumunun analizi ile birlikte bu sistemlerde uygulanan sığır refahı standartlarının hedefleri ve etkinliklerinin karşılaştırılması amaçlanmıştır.
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