The aim of this work is the isolation, identification and evaluation of the antibacterial activity of endophytic fungi from the Algerian medicinal plant Marrubium vulgare L. The isolation of endophytic fungi is carried out by the method of sterilization of surface to eliminate epiphytes then incubation of the fragments treated according to a precise protocol. The identification of the grown isolates allowed us to obtain three fungal strains belonging to three genera: Fusarium, Alternaria and Chaetomium, which are generally of the Deuteromycete phylum. The antibacterial capacity of the fungi was tested against five human pathogenic bacteruim using the agar-fungi disk diffusion method. With an inhibition zone (IZ) spanning from 7.5 to 25 mm, all isolated fungal strains showed antibacterial activity against at least one of the bacteria tested.. However, Fusarium sp has the highest antibacterial activity with an IZ of 19 and 24 mm against S. aureus and B. subtilis, respectively. Finally, our results clearly confirm that the medicinal plant M. vulgare L. presents a reservoir of endophytic fungi, which can be used in various fields, especially pharmaceutical fields.

The Litsea floribunda Gamble was widely used for various medicinal purposes. The present study was aimed to screening the phytochemicals presentin theleaf and bark extract of L. floribunda and also assess their antioxidant and anti-inflammatory properties. The results revealed that the leaf and bark extract showed the extracts are rich in phytomoleculeslikephenolics and tannin. The leaf extract showed more antioxidant activitiessuch as total antioxidant, ABTS, superoxide radical scavenging, FRAP, and DPPH reducing power activities were done respectively. Better radical activities Albumin denaturation and Anti-proteinase were observed in leaf extractthan in the bark extract, also it showed significant anti-inflammatory activities. Finally, L. floribunda could be considered as the most valuablealternative medicinalsource for many diseases.

The storage of spermatozoa and keeping alive for years in the spermatheca by the honey bee queen is a phenomenon allowing her to fertilize eggs throughout her life. In this study, the queens that were 2-week, 1-year, and 2-year-old were analyzed to determine the viability of spermatozoa in their spermathecae. The sister queens reared by the grafting method were instrumentally inseminated with 8 μl fresh semen when they became 6 days old. One week after instrumental insemination, one batch of queens (2-week-old) was dissected for spermatozoa viability test. Another set of queens was introduced into production colonies in Langstroth hives after the onset of oviposition in the mating nuclei. The queens were maintained in production colonies for one year and two years until the dissection process for spermatozoa viability test. The viability of spermatozoa was measured by the dual staining method. We determined that the mean viability of spermatozoa in 2-week, 1-year and 2-year-old queens were 97.3%, 91.1% and 88.1%, respectively. The viability of spermatozoa in queens decreased with age, and the differences between the viability means were significant. However, we did not detect a steep decline in the viability of spermatozoa in queens (6% in one year and 9% in two years) in a wide range of timescale in contrast to previous reports. Furthermore, we found high viability of spermatozoa in the spermathecae of queens at the start of their lives and one- and two-years of age.

Rice (Oryza sativa L.) is the most important staple cereal crop which is consumed by more than 50% of world population. It contributes 23% and 50% of total calories consumed by world and Nepalese population respectively. Among various abiotic factors affecting rice, rice blast is the most disastrous, causing 70-80% yield loss. This disease was originated in China around 7000 years ago. In Nepal, it was first reported in Thimi, Bhaktapur in 1966. It is caused by a filamentous ascomycete fungus Magnaporthe oryzae (Anaemorphic form- Pyricularia oryzae). It infects all the developmental stage of plant and produce symptoms on the leaf, collar, neck, panicle and even in the glumes. It decreases the rice production by an amount, enough to feed 60 million people every year. Cloudy weather, high relative humidity (93-99%), low night temperature (15- 20°C), longer duration of dew is the most favorable condition for the outbreak of disease. The most usual approaches for the management of rice blast diseases are management in nutrient fertilizer and irrigation, application of fungicides and plantation of resistant cultivars. Besides, the use of extracts of C. arabica are reported to have an inhibitory effect on the disease. Seed treatment with Trichoderma viridae @ 5ml/lit of water have also been found effective. The chemical means of controlling blast disease shall be reduced, instead eco-friendly measures like biocontrol agents, resistant varieties, plant extracts can be practiced for disease control. Different forecasting model can be used in order to predict the disease prevalence.

The main purpose of this study is to compare the perceived stress level of university students in terms of body-mass index categories and gender. 235 university students (183 female, 53 male) between the ages of 18-40 participated in the study. Participants were selected from six different departments of six universities by convenient sampling. In the study, a personal information form asking about university, department, age, disease status, drug use, weight, height and gender, and a perceived stress scale were used as data collection tools. While body mass index (BMI) categories and gender were determined as independent variables in the study, perceived stress level was determined as dependent variable and the data were analysed with a one-way ANOVA test. The findings revealed that there was no statistically significant difference in the perceived stress levels of the participants in terms of gender and body mass index categories. However, for one factor of the stress scale (readiness to cope with stress), participants in the normal body mass index range reported significantly less stress than participants in the obese body mass index range. The findings will be discussed in the light of the literature.

Heavy metal pollution generally occurs due to socio-economic, industrial, and anthropogenic activities, which may cause an environmentally hazardous and serious severe threat to the survival of the organisms (genotoxic, carcinogenic, and clastogenic effects on it). Many physical and chemical remediation approaches have been proposed to deal with this pollution, but these are very time-consuming and costly. While bioremediation stands out as an inexpensive and efficient approach, the use of bacteria is thought to be a potential and productive organism to prevent this pollution. This review has evaluated the bacterial potential to clean up heavy metals from the environment and elucidated the mechanisms responsible for bioremediation.

The gastrointestinal parasitic infestation seems to have a substantial economic impact on the entire livestock production industry. Because of the parasites' environmental appropriateness, numerous domestic animals in Bangladesh are particularly susceptible to the infestation; however, the extent to which people are aware of the potential risk factors is debatable. Therefore, the current meta-analysis' objective is to determine the precise understanding of potential risk variables and the impact of climatological variations on five important gastrointestinal parasites: Paramphistomum species, Strongyloides species, Trichuris species, Schistosoma species, and Moneizia species. Four globally recognized databases, including Web of Science, Scopus, PubMed, and Google Scholar were screened to choose the studies published in English language from 2000 to 2020. Finally, 29 studies were selected for further analysis and recorded the maximum prevalence in Paramphistomum species (26%; 95% CI: 19-33), followed by Strongyloides species (9%, 95% CI: 5-12), Trichuris species (10%, 95% CI: 4-17), Schistosoma species (19%, 95% CI: 7-31), and Moneizia species (8%, 95% CI: 5-10); besides the overall prevalence was noted as 15% (95% CI: 11-18). Furthermore, subgroup analysis revealed that parasite infestation was most prevalent in females (21%) and the elderly animal population (14%) and the summer season (26%). To sum up, the current meta-analysis visualized the epidemiological risk factors with the overall incidence of five major parasite infestations in livestock animals in Bangladesh; hence, the government and shareholders may employ it as proof before launching any control programs or improving farmers' awareness.

Roasting is the most important coffee processing stage that affects coffee flavour. Coffee brew is prepared by various methods and Turkish coffee brewing technique is one of the oldest methods among other coffee preparation techniques. In this study, volatile compounds of light, medium, dark roasted ground coffee and Turkish coffee brews prepared from light, medium, dark roasted coffees were evaluated and the effect of roasting process was determined. The volatile compounds from acid (2), alcohol (1), aldehyde (2), furan (8), furanone (3), ketone (1), lactone (1), phenol (3), pyrazine (19), pyridine (1), pyrrole (6), and thiophene (1) chemical group was detected in all roasted ground coffee samples. However, volatiles compounds from acid, alcohol and furanone group were not detected in any Turkish coffee brew. Pyrazines were main volatile compounds of light roasted ground coffee and Turkish coffee brew of light roasted coffee. On the other hand, furans were main volatile compounds of medium, dark roasted ground coffee and Turkish coffee brew prepared from medium, dark roasted coffees followed by pyrazines.

Tea is a popular beverage with potential health benefits for those who consume it regularly. Tea products with health claims require scientific evidence based on clinical trials with generally accepted scientific data and newly developed scientific data. Tea products have been labelled FOSHU in Japan, claiming to reduce body fat and cardiovascular risk. In The USA, health claims on tea cannot be recommended and categorized as Qualified Health Claims. In Europe, health claims for tea still required further research to provide accepted scientific data. This review aims to explain the position of tea products based on the perspective of the regulatory framework of food health claims in the different official agencies in the United States, Europe, and Japan.

Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties.