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Safety Assessment of Dairy Microorganisms, Streptococcus thermophilus and Lactobacillus bulgaricus, Isolated from Traditional Yoghurt Cultures Full text
2018
Yekta Gezginç | Fatma Gül Demirbanka | Elif Coşkun Dağgeçen | İsmail Akyol
The traditional fermented food consumption has become very popular because of the increasing public concern about food additives. Lactic Acid Bacteria (LAB) species have traditionally been used as starter cultures in the production of fermented food. LAB can acquire antibiotic resistance from other bacteria in the natural environment and different resistant mechanisms via mutation. The resistance of bacteria to antibiotics is an increasingly important public health problem worldwide. In this study, antibiotic resistance of 115 Streptocoocus thermophilus and 35 Lactobacillus bulgaricus isolates obtained from traditional Turkish yogurts were tested against kanamycin, chloramphenicol, erythromycin, ampicillin, rifampicin, tetracycline, vancomycin and gentamicin using disc diffusion method. Study results showed that most strains were susceptible to all the antibiotics tested while a few of them were determined to be resistant only to kanamycin, ampicillin, erythromycin, and tetracycline. When contacted in a human body, resistant strains might transfer the related genes to the pathogenic species, which may result in devastating consequences.
Show more [+] Less [-]Karabuğday: Bileşimi ve Gıdalarda Kullanılması Full text
2018
Su Kılıç | Yeşim Elmacı
Karabuğday (Fagopyrum esculentum Moench ve Fagopyrum tataricum (L.) Gaertn), bileşiminde yüksek oranda protein, diyet lif, vitamin, mineral madde, temel çoklu doymamış yağ asitleri bulundurmakta, başta rutin olmak üzere önemli antioksidanlar ve fenolik bileşenler için kaynak oluşturmaktadır. Protein biyo-yararlılığının yüksek olmasının yanı sıra gluten içermemesi nedeni ile çölyak hastalarının beslenmesine uygun, besleyici değeri yüksek önemli bir alternatif olmaktadır. Karabuğday, çeşitli yöresel ürünlerde ham bileşen olarak yer alıp insan beslenmesindeki yerini günümüze kadar korumuştur. Beslenmedeki öneminin ve sağlık üzerine olumlu etkilerinin son yıllarda yeniden keşfedilmesiyle birlikte beslenmede ve birçok gıda formülasyonunda önemli bir bileşen olarak artan bir popülerlik ile kullanılmaktadır. Bu çalışmada, karabuğdayın kimyasal bileşimi ve karabuğday kullanılarak geliştirilen gıdalar hakkında bilgi verilmiştir.
Show more [+] Less [-]Investigation of Some Immunological Parameters in Rainbow Trout (Oncorhynchus mykiss) Injected Propolis Full text
2018
Muhammet Enis Yonar | Naim Sağlam | Seval Yılmaz
In this study, effects of propolis on immunological parameters of rainbow trout were investigated. For this purpose, propolis was intraperitoneally injected to fish 4 times at doses of 2.5, 5 and 10 mg / kg fish weight. Blood samples on 3, 9, 15 and 21 days were collected from fish of the control and experimental groups and analysed to determine oxidative radical production [nitroblue tetrazolium (NBT) activity], total plasma protein and total immunoglobulin levels. At the end of the experiment, there was a statistically significant increase in oxidative radical production, total protein and immunoglobulin levels of propolis treated groups when compared to the control group.
Show more [+] Less [-]Determination of Suitable Extraction Method for the Available Iron (Fe) Content of Calcareous Soils Full text
2018
Aydın Adiloğlu | Sevinç Adiloğlu | Mehmet Rüştü Karaman
The aim of this research was to determine the most suitable extraction method for the available iron contents of calcareous soils in Trakya Region, Turkey. For this purpose ten calcareous soil samples were taken from research area and five extraction methods (Lindsay and Norvell, Wear and Evans, Olson, Soltanpur and Mehlich methods) were used and three biological indices (dry matter yield, Fe concentration, Fe uptake) were compared. The plant biological indices were determined with wheat (Triticum aestivum L.) plant grown under greenhouse conditions. At the end of the experiment, the highest correlation coefficients (r) were determined between the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA, 0.005 M DTPA + 1 M NH4HCO3 methods and the biological indices. The correlation coefficients (r) for the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA method and the three biological indices were 0.648**, 0.780** and 0.656** respectively. For the 0.005 M DTPA + 1 M NH4HCO3 method, these coefficients were determined 0.595**, 0.637** and 0.625**, respectively. Consequently, these extraction methods were suggested for the determination of the available Fe contents of the calcareous soils in Trakya Region, Turkey.
Show more [+] Less [-]Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria Full text
2018
Flora Oluwafemi | Bosede Folashade Oluwabamiwo | Oluseyi Oluwajubelo Oluwatosin | Olufunmilayo Ruth Akinrinade | Adelodun Lawrence Kolapo
This study investigated the prevalence and concentration of antibiotic residues in twenty brands of milk. Determination and quantification of antibiotic residues was done using High Performance Liquid Chromatography. Aerobic plate count was carried out and the associated bacteria isolated. Identification of bacteria was done using standard microbiological methods. Molecular characterization and identification of bacterial isolates was done using 16S rRNA gene sequencing method. Antibiotic sensitivity was carried out on bacterial isolates using disc diffusion method. Plasmid profile of drug resistant isolates was done using alkaline analysis method. Comparison of means was done using Analysis of Variance. Antibiotic residues were detected in 10 (50%) of the 20 milk brands analyzed. The residual levels of tetracycline, oxytetracycline and chlortetracycline ranged between 5ng/kg and 1569ng/kg while none of the samples had doxycycline residues. The prevalence of tetracycline residues in evaporated milk brands and powdered milk samples were 100 and 23 % respectively. The aerobic plate counts ranged from 2.5 × 102 to 6.5 × 102 CFU∕mL for evaporated milk and 2.5 × 101 to 6.0 × 101 CFU∕mL for powdered milk. A total of ten bacterial species were isolated and identified. Susceptibility result showed that 95% of the isolates were sensitive to erythromycin, cefuroxime and gentamycin; 12.5% were resistant to tetracycline and ampicillin, 10% were resistant to ofloxacin and 7.5% to doxycycline and penicillin. Five of the bacterial isolates were resistant to more than one class of antibiotics. Resistant isolates subjected to plasmid profiling had detectable plasmids with estimated sizes between 120bp and 1000bp. The obtained results provide evidence that the presence of antibiotic residues in evaporated and powdered milk is an indication that the public is exposed to the harmful effects of the residues.
Show more [+] Less [-]Evaluating the Effectiveness of Different Rhizobia Strains and Their Effect on Crop Yields in Acid Soils of Western Kenya Full text
2018
Janet Kemuma Ogega | Beatrice Ang’iyo Were | Abigael Otinga Nekesa | John Robert Okalebo
Food insecurity in Sub - Saharan Africa (SSA) is on the rise due to soil fertility depletion and in Kenya, Nitrogen (N) is one of the widely deficient nutrients. Biological nitrogen fixation (BNF) can replenish N into the soil system. A study was carried out in acid soils at Koyonzo and Ligala sites of western Kenya to determine the effectiveness of different inoculants after agricultural lime application in enhancing BNF and yields of groundnuts (Arachis hypogea L.) and maize (Zea mays L.) intercrop. Red Valencia groundnut variety was intercropped with Hybrid 513D maize variety. A6w, W1w and V2w indigenous rhizobia strains were tested alongside a commercial rhizobia strain called biofix. Nitrogen treatment was included as a positive control. The results showed that inoculation significantly increased nodule number and weight per plant. There were significant differences among indigenous rhizobia in fixing N. Rhizobia inoculation accounted for 58.91% and 78.95% increase in the amount of N fixed above the control at Koyonzo and Ligala respectively. The strain that fixed the highest amount of N was A6w followed by V2w and W1w at both sites under the dolomitic soil amendment with the values of 14.67, 9.56, 3.53 and 11.37, 8.20 and 1.50 kg N ha-1, respectively at Koyonzo and Ligala sites. Rhizobia inoculation accounted for 80.96% and 47.09% maize yield increase at Koyonzo and Ligala respectively. The best inoculant A6w, gave maize yields of 3.76 and 2.78 t ha-1 at Koyonzo and Ligala, respectively. In conclusion soil amendment with dolomitic lime and inoculating groundnuts with rhizobia strain A6w resulted in increased groundnut and maize yields. This practice can, therefore, be adopted by farmers in western Kenya to improve the productivity of the groundnut maize intercropping systems.
Show more [+] Less [-]The Effects of Age and Number of Grafted Larvae on Some Physical Characteristics of Queen Bees and Acceptance Rate of Queen Bee Cell Full text
2018
Samet Okuyan | Ethem Akyol
This study was carried out to determine the effects of age and number of grafted larvae on acceptance rates, body weight, body length, head width and length, thorax width and length, and wing width and length of queen bees. One breeding and eleven starter hives were used for rearing queen bees. Totally, 495 one, two, and three-day-old larvae were grafted into starter hives comprising 30, 45, and 60 and 414 larvae were accepted; thus, generally, the acceptance rate was calculated as 83%. The best acceptance rate was calculated in three-day-old larvae group as 85.15%. There were found a statistically significant effect of the number of grafted larvae on body length and head width of queen. However, there were any effects on weight, head length, thorax width, thorax length, wing width, wing length, and acceptance rate of larvae. Age of grafted larvae did not have a statistically significant effect on head width, head length, wing width and acceptance rate of larvae. On the other hand, age of grafted larvae had a statistically significant effect on queen weight, body length, thorax width and length, and wing length of queen. If bee breeders wish to improve their stock, they should graft one-day-old larvae for rearing better queen bees.
Show more [+] Less [-]Recycling Model Suggestion for Sustainable Urban Green Areas in Konya Full text
2018
Filiz Çelik
Urban green spaces can be a comprehensive tool to ensure the sustainability of cities. The sustainability of green areas in planning, design, implementation, maintenance and operation processes contributes to the sustainable development of cities as well. There are many ways to ensure that green areas are sustainable from a social, economic and ecological point of view. These include the construction and use of compost under recycling and re-evaluation. The wastes generated as a result of periodic maintenance in urban green areas are disposed together with other wastes in solid waste storage area. However, the use of organic wastes in composting can ensure that green areas are sustainable by reducing both recycling and periodic maintenance costs of green areas. The aim of this study is to improve the idea of recycling and re-evaluation of organic wastes which are emerged in green areas by composting and using them in green areas. For this purpose, a model for composting and replacing fertilizer from organic wastes emerged in green areas of Konya was proposed. Thus, the costs of green areas with very high design, implementation and maintenance expenses will be reduced and they will be sustainable within themselves. The sustainability of urban green spaces will also contribute to Konya's urban sustainability.
Show more [+] Less [-]Some New Weed Control Methods in Organic Farming Full text
2018
Taner Yıldız | Fikret Özkaraman | Dilek Kandemir
The chemicals used for weed control cause pollution at both ground and surface waters, and lead to accumulation of pesticide residues in vegetables, fruits and field crops. For this reason, avoiding of use of chemicals and instead applying natural production methods (organic farming) emerged as a people desire. In recent times, the environmental consciousness has increased rapidly all over the world and potential dangers related to use of chemicals led scientists and people to worry. There have been extensive studies on different methods and machines, which can be used for weed control, as they do not leave any residues at soil and water sources. In this study, some new weed control methods were included that can be applied in organic farming.
Show more [+] Less [-]Isıtma İşleminin Çekişte Zeytinyağının Kalite, Oksidatif Stabilite ve Yağ Asidi Bileşimine Etkisi Full text
2018
Aslı Yıldırım | Derya Deniz Şirinyıldız | Şeyma Nur Akkaya | Ezgi Genç | Aslı Yorulmaz
Çalışmanın amacı Çekişte çeşidi zeytinlerden elde edilen zeytinyağlarının ısıtma işlemi sırasında temel kalite kriterleri, oksidatif stabilite ve yağ asidi bileşiminde meydana gelen değişimlerin belirlenmesidir. Bu amaçla Aydın ilinden 2017 yılında hasat edilmiş olan Çekişte çeşidi zeytinlerden elde edilmiş olan zeytinyağı, 100°C sıcaklıkta ve 20 L/saat hava akışı altında toplamda 50 saat süreyle ısıtılmıştır ve 10 saatlik aralıklarla serbest yağ asidi oranı, peroksit sayısı, dien ve trien (K232 ve K270) değerleri, toplam fenol miktarı, antioksidan aktivite, klorofil ve karotenoid miktarları ile yağ asidi kompozisyonları yönünden analiz edilmiştir. Ayrıca örneklerin indüksiyon periyodu olarak ifade edilen oksidatif stabiliteleri belirlenmiştir. Çalışma sonunda temel kalite parametrelerinden serbest yağ asidi miktarı, peroksit sayısı, dien ve trien (K232 ve K270) değerlerinin ısıtma işlemi ile birlikte artış gösterdiği saptanmıştır. Örneklerin toplam fenol, antioksidan aktivite, klorofil ve karotenoid miktarları ise ısıtma süresinin artmasıyla birlikte kademeli olarak azalmıştır. Benzer şekilde indüksiyon periyodunun da işlem süresinin artmasıyla birlikte azaldığı tespit edilmiştir. Zeytinyağı örneklerinde tespit edilen yağ asitlerinden linolenik asit miktarının ise işlem sonunda önemli düzeyde arttığı, oleik asit miktarının ise işlem öncesine göre azalma eğiliminde olduğu saptanmıştır.
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