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Biochemical Responses of Shoot and Root Tissues of Sainfoin (Onobrychis viciifolia Scop.) to NaCl-Salt Stress Under İn Vitro Conditions Full text
2019
Ramazan Beyaz
Sainfoin (Fabaceae) is one of the most critical animal forage crops. However, the tolerance of sainfoin is low against to salinity. This study aims to investigate biochemical responses of the shoot and root tissue of sainfoin seedlings to moderate salt stress under in vitro conditions. For this aim, the seed of sainfoin were sown MS medium containing 100 mM NaCl. Antioxidant enzymes (CAT, SOD, APX, and GR), proline and malondialdehyde (MDA) contents were measured in shoot and root tissue of 35-day-old seedlings of sainfoin. A significantly higher constitutive catalase (CAT) and superoxide dismutase (SOD) activity was observed in shoot tissues when compared to root tissues. Overall, salt stress caused significant more enhancement in the activity of antioxidant enzymes (CAT, SOD, APX, and GR) in shoot tissues than root tissue. On the other hand, among the antioxidant enzymes, SOD seems to be more active in both tissues of sainfoin. Interestingly, the activity of GR reduced in both tissue under salt stress. The content of proline and MDA has been increased under salt stress and this increase has been more in the root tissue. This study has revealed biochemical responses to salt stress in different organs of sainfoin.
Show more [+] Less [-]Effects of Different Sowing Densities on Some Agronomic Characteristics of Camelina (Camelina sativa L.) Full text
2019
Güngör Yılmaz | Şaziye Dökülen | Ahmet Kınay
This research was carried out to determine the appropriate sowing density in camelina in Tokat ecological conditions. The study was carried out using Ames 26680 camelina genotype in 2016 and 2017 for two years. In this study, 10, 20, 30 and 40 cm row spacings were used. Plant height (cm), number of branches per, number of capsules per plant, number of seed / capsule, thousand seed weight (g), seed yield (kg/da), oil ratio (%) and oil yield (kg/da) were investigated. According to the results, the seed yields of the two years were significantly different. The highest yields were obtained from 20 cm (132,0 kg/da) in 2016 and from 40 cm row spacing (130,6 kg/da) in 2017. As the average of two years, yields from 20, 30 and 40 cm row spacings did not differ significantly. As the average of two years, the oil rate was 36,2% and the oil yield was 43 kg/da. Thus, it is concluded that in fall sowing under Tokat conditions, camelina can be sown in 20 to 40 cm row spacing using 10 kg seed per hectare.
Show more [+] Less [-]Physicochemical Properties of Cheddar Cheese made from Citrus reticulata Blanco Crude Flowers Extract Full text
2019
Usman Mir Khan | Ishtiaque Ahmad | Saima Inayat | Hafiz Muhammed Arslan Amin | Zeliha Selamoglu
Citrus reticulata Blanco crude flowers extracts (CFE) at four different concentrations (1, 2, 3 and 4%, v/v) were used as natural milk coagulant instead of rennet to apply for Cheddar cheese making from buffalo milk. The physicochemical properties of Cheddar cheeses were compared with cheese made with 0.002% (v/v) rennet (control cheese). Physicochemical properties of Cheddar cheese showed that cheese made with 1% and 2% of CFE had a crumbly and slightly softer texture/appearance. While cheeses containing 3 and 4% crude flowers extracts had semi-hard texture/appearance of curd similar to rennet added cheese. Protein analysis shows that crude flowers extracts made cheese had significantly higher protein content than control. The Cheddar cheese with 3% and 4% CFE were preferred by panelists instead of 1% and 2% CFE for their taste, texture/appearance and overall acceptability. Conclusively, crude flowers extracts coagulated Cheddar cheese fulfills the compositional requirement with acceptable organoleptic characteristics and at the same time provides nutritional health benefits.
Show more [+] Less [-]Cover and Table of Contents Full text
2019
Editoral Editoral
Artan Dozlarda Kurşun (Pb) Uygulamalarının Tütün Çeşitlerinin Büyüme, Pb ve Mikro Element Konsantrasyonlarına Olan Etkileri Full text
2019
Ahmet Kınay | Halil Erdem
Kurşun (Pb), belirli eşik konsantrasyonları aştığında insanlar, hayvanlar ve bitkiler için tehlikeli olan bir ağır metaldir. Tütün bünyesinde yüksek konsantrasyonlarda kurşun biriktirebilen bir bitkidir. Kurşun alımında tütün genotipleri arasındaki farklılıklar ve bitkilerin kurşuna verdikleri henüz cevap açıkça belirlenmemiştir. Bu çalışmanın amacı, Pb dozlarının (Pb 0; 0,25; 2,5 ve 10 mg kg-1) iki farklı tütün çeşidinin (Xanthi/2A ve Nail) biyomas (kök ve yeşil aksam) üretimi, Pb konsantrasyonu ve mikro besin konsantrasyonları üzerindeki etkilerini belirlemektir. Kontrollü koşullar altında yetiştirilen tütün çeşitlerine artan Pb dozları ile birlikte temel makro (N, P ve K) ve mikro (Fe ve Zn) besin elementleri verilmiştir. İki farklı tütün çeşidinde biomas üretimi (kök ve yeşil aksam kuru madde verimi), yeşil aksam Pb, Zn, Fe, Mn ve Cu konsantrasyonları belirlenmiştir. Artan dozlarda Pb uygulaması ile çeşitlerin kuru madde verimi önemli ölçüde etkilenmiştir. Kök ve yeşil aksamda ortaya çıkan azalmaya karşın, artan dolarda kurşun uygulamaları ile tütün çeşitlerinin yeşil aksam kurşun konsantrasyonlarında istatiksel olarak önemli artışlara neden olduğu ortaya çıkmıştır. Artan dozlarda Pb uygulaması ile tütün çeşitlerinin yeşil aksam Zn, Fe, Mn ve Cu konsantrasyonlarında istatiksel olarak önemli artış ve azalışlara neden olduğu ortaya çıkmıştır. Sonuç olarak en yüksek kurşun dozunda dahi Nail ve Xanthi/2A tütün çeşitlerinin yeşil aksamında 1,0 mg kg-1’ın altında Pb konsantrasyonuna sahip olduğu ortaya çıkmıştır. Buna rağmen kurşun ile kirlenmiş topraklarda yetişen tütün çeşitleri ile yapılan tütün mamulleri insanlar içim risk teşkil etmektedir.
Show more [+] Less [-]Acetylcholinesterase, Specific Acetylcholinesterase and Total Protein Concentrations in the Brain Regions of Broiler Chickens Fed Dietary Monosodium Glutamate Full text
2019
Olumuyiwa Joseph Olarotimi | Imoleayo Sarah Oladeji | Olufemi Adesanya Adu | Francis Ayodeji Gbore
The study was carried out to examine the effect of varied levels of dietary monosodium glutamate on acetylcholinesterase, specific acetylcholinesterase and total protein concentrations in the brain regions of broiler chickens. Three hundred (300) day – old unsexed Abor – acre chickens were randomly assigned to diets: A, B, C, D, E and F containing 0.00, 0.25, 0.50, 0.75, 1.00 and 1.25 g/kg MSG respectively. Each treatment was replicated 5 times with 10 birds per replicate. The birds were fed ad – libitum and provided with clean water for 8 weeks (56 days) after which 2 birds per replicates were slaughtered. The brains were removed, dissected into different regions comprising of the olfactory lobe, pineal body, optic lobe, cerebellum and the medulla oblongata. The different parts of the brain were homogenized to determine the acetylcholinesterase and total protein which were also used in the assessment of the specific acetylcholinesterase of the brain. No significant differences were observed in the acetylcholinesterase activity of the olfactory lobe, pineal body, optic lobe, cerebellum except for the medulla. Likewise, the dietary monosodium glutamate did not influence the activities of the total protein and specific acetylcholinesterase of the olfactory lobe portion of the brain. The dietary monosodium glutamate exerted significant effects on the total protein of other brain parts studied and which invariably resulted in significant changes in the specific acetylcholinesterase of the optic lobe, cerebellum and medulla except for the optic lobe. This study revealed that monosodium glutamate added to broilers diet above 0.75 g/kg significantly altered the concentration of the brain acetylcholinesterase, total protein and specific acetylcholinesterase thereby impaired brain functions.
Show more [+] Less [-]Some Chemical Properties of Infrared Dried Neem Fruit in Turkey Full text
2019
Tunahan Erdem
In Turkey, the Rosary (Neem) tree, known as the Melia azedarach L. is a type of evergreen plant. In the world, four different species of tree grows native in India, Burma, Pakistan, South Asia, and Australia. In our country, the Neem tree (Melia azedarach L.) grows naturally in tropical zones with light yellow fruit and green leaves. Fruits can reach maturity in September-October morphologically. Neem oil from fruits and powder from fruits and leaves are the main products which are traded in abroad as organic substances. In this study, neem fruit was investigated to obtain the neem oil from Melia azedarach L. in details such as moisture content (MC), drying rate (DR), moisture ratio (MR), Azadirachtin amount (AZ) and macro and microelement parameters. The fruits were collected from locally Turkey and de moisturized in the greenhouse for one week than dried in Infrared cabinet dryer to obtain the neem oil. The Azadirachtin amount results were found 46.1; 45.4; 48.4 (mg/g) through three replications.
Show more [+] Less [-]Correlations of Animal Welfare Scores with First Lactation Milk Yield of Dual Purpose Cows Full text
2019
Cigdem Durmaz | Savas Atasever
The objective of this study was to determine the relationships between animal welfare scores (AWS) and milk production in dairy cattle. AWS traits on locomotion ability (LA), social interaction (SI), flooring (F), indoor conditions (IC) and stockmanship effect (SE) were scored by a 100 points scale in a total of 51 farms in Samsun region of Turkey. First lactation milk yield (FLMY) records of Brown Swiss and Simmental cows were used as milk production trait. All components were assessed by t-test to determine the effects of the welfare traits on FLMY. The overall mean of AWS was calculated to be 74.05±1.69, and therefore the farms were founded within a “suitable” class. It was estimated moderate correlations among the parameters except for AWS and SI, however, no statistically significant difference was estimated between AWS and FLMY of the cows. SE was revealed the most important factor on AWS of the farms. Finally, routine scoring cattle farms to decide their suitability for animal needs is advised to herd owners for managing rentable populations in the farms.
Show more [+] Less [-]Contribution to the Lepidoptera Fauna of Hatay (Turkey) Full text
2019
Erol Atay | Mahmut Tatlı
Field studies were carried out in the localities of different altitudes and vegetation covers in the Hatay province in March-October 2018. Papilio demoleus was caught with insect net in the citrus orchards at Antakya and Reyhanlı. Cornifrons ulceratalis, Macaria alternata and Camptogramma bilineatum were caught mercury vapour light trap and white screen in different localities, at different elevations, in different climatic conditions, plant cover and sutface features in Erzin. Papilio demoleus was caught in the citrus gardens in the province of Hatay Reyhanlı district as a male and a female on 05.09.2018 and in Antakya district on 15.10.2018 as two male and three female individuals. This invasive species is widespread all over the world and has the potential to create substantial threat for citrus orchards in the Mediterranean region and in the eastern Mediterranean region of Turkey in next years. As a result, Papilio demoleus (Papilionidae), Cornifrons ulceratalis (Crambidae), Macaria alternata and Camptogramma bilineatum (Geometridae) are new records for the lepidoptera fauna of Hatay.
Show more [+] Less [-]Patlıcanda In vitro Rejenerasyon Ortamında Farklı Orizalin ve Kolhisin Konsantrasyonlarının Tetraploid Bitki Üretimine Etkisi Full text
2019
İlknur Çeğil | Sebahattin Çürük
Bu çalışmanın amacı, Faselis F1 ve Karnaz F1 patlıcan çeşitlerinde kolhisin ve orizalinin in vitro rejenerasyon ortamında kullanılmasıyla tetraploid bitki elde etmektir. Araştırmada, yaprak eksplantları 10 µM BA ve 1 µM IAA içeren katı MS rejenerasyon ortamında 5 ve 7 gün bekletilmiştir. Ardından bu eksplantlara kolhisinin 2,5 ve 3,75 mM konsantrasyonları 8, 16 ve 32 saat süreyle; orizalinin ise 28,8 ve 43,2 µM konsantrasyonları 12, 24 ve 36 saat süreyle uygulanmış ve tekrar kolhisinsiz ve orizalinsiz rejenerasyon ortamına aktarılmıştır. Rejenerasyon ortamında oluşan kallus, tomurcuk veya kısa sürgünler 0,5 µM BA ile desteklenmiş MS ortamına alınarak bitkilerin oluşması sağlanmış ve ploidi seviyesi flow sitometri ile belirlenmiştir. Karnaz F1 çeşidinin eksplantlarına 3,75 mM kolhisin dozunun uygulaması sonucu, 2,5 mM’a göre daha yüksek tetraploid bitki oluşumu sağlanmıştır. Bu çeşitte uygulanan orizalin denemesinde ise en yüksek tetraploid bitki oluşumu, eksplantların 7 gün rejenerasyon ortamında bekletilmesinden sonra 43,2 µM orizalin konsantrasyonunun 24 saat uygulamasından elde edilmiştir. Karnaz F1 çeşidinde kolhisin ve orizalin uygulamasından elde edilen tetraploid bitkilerde çiçek tozu canlılığı %76,99 ve %81,19, çimlenme %19,14 ve %17,98 olarak gerçekleşmiştir. Faselis F1 çeşidinin rejenerasyon ortamında 7 gün bekletilen yaprak eksplantları 2,5 mM kolhisin konsantrasyonunda 8 ve 32 saat inkübe edildiğinde, diğer uygulamalara göre daha yüksek tetraploid bitki elde edilmiştir. Aynı çeşidin orizalin denemesinde, eksplantların 5 gün rejenerasyon ortamında bekletilmesi sonucu daha yüksek tetraploid bitki üretilmiştir. Faselis F1 çeşidinde kolhisin ve orizalin uygulamaları sonucu üretilen tetraploid bitkilerde çiçek tozu canlılığı %86,41 ve %95,68, çimlenme ise %26,54 ve %28,47 olarak belirlenmiştir.
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