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Detection of Prunus Necrotic Ringspot Virus (PNRSV) and Apple Mosaic Virus (ApMV) in Rose (Rosa spp.) Plants in Konya Province Full text
2025
Adile Tuğçe Orhan | Serkan Yeşil
This research was conducted to determine the presence of Prunus necrotic ringspot virus (PNRSV) and Apple mosaic virus (ApMV) that can cause infection in rose (Rosa spp.) plants grown in Konya province. For this purpose, field and laboratory studies were carried out in areas where roses are mostly grown for landscaping purposes, and infection rates were calculated with the data obtained. The hypothesis of the study is that roses in Konya province may be infected with PNRSV and ApMV and the presence of these viruses can be determined. In line with this hypothesis, various rose growing areas in Konya province were selected as the research area. During the field studies in 2023, guided sampling was carried out and 94 leaf, branch and flower samples were collected from different rose varieties. The collected samples were tested for determining of PNRSV and ApMV infections by Double Antibody Sandwich (DAS) ELISA method, which is one of the serological testing methods in the laboratory. As a result of the tests, it was revealed that the single infections of PNRSV and ApMV were present in 12 and 11 samples of rose plants in Konya province. The total infection rate of both viruses in the province was calculated as 24.47%. Also, PNRSV+ApMV mixed infections were detected in 2 samples. In this study, the infections of PNRSV and ApMV on roses in Konya province were determined for the first time by serological methods. These results will serve as an important source of information for rose producers and agricultural engineers in the region and will allow the development of strategies to control the spread of viruses and minimize infections.
Show more [+] Less [-]Determination of Germination and Emergence Performance of Hemp (Cannabis sativa L.) Seeds at Different Maturity Stages Full text
2025
Güngör Yılmaz | Cebrail Yıldırım | Nurullah Dursun Gür
Seed maturation in hemp is not homogeneous, and the harvest is done when the seeds are 70-80% mature. This study was carried out to determine the germination and emergence performance of hemp (Cannabis sativa L.) seeds at different maturation stages. Mature, semi-mature and immature seeds were used as material in the study. The study was carried out as two separate experiments, germination and emergence. The germination test was carried out in petri dishes and the emergence test in viols. Both experiments were carried out according to the randomized complete block design with 3 replications. Germination rate (%), shoot and root length (mm), shoot and root fresh weight (mg plant-1) and shoot and root dry weight (mg plant-1) parameters were investigated in the germination study. In the emergence study, the emergence rate (%), seedling and root length (mm), seedling and root fresh weight (mg plant-1), and seedling and root dry weight (mg plant-1) values were examined. In the germination study, semi-mature seeds germinated but did not develop. Therefore, no data could be obtained from other parameters except germination rate. In germination study, germination rate varied between 6.67 and 84.67%, shoot length 27.2-38 mm, root length 39.4-50.8 mm, shoot fresh weight 30.66-49.89 mg plant-1, root fresh weight 4.32-7.69 mg plant-1, shoot dry weight 3.25-7.99 mg plant-1, root dry weight 0.68-2.03 mg plant-1. In the emergence study, emergence rate ranged between 5.18-82.69%, shoot length 68-136.4 mm, root length 37.4-69.6 mm, shoot fresh weight 60.33-154.80 mg plant-1, root fresh weight 27.30-46.73 mg plant-1, shoot dry weight 3.97-10.24 mg plant-1, root dry weight 2.61-5.43 mg plant-1. In both experiments, the highest values obtained from all the examined traits were obtained from mature seeds. In both studies, the highest values were obtained from mature seeds for all traits examined. Semi-mature and immature seeds gave similar results in terms of the traits examined.
Show more [+] Less [-]Will Olive Groves have a Future Under Climate Change Conditions in The North Aegean Sub-Region, a Mediterranean Agricultural Ecosystem of Türkiye? Full text
2025
Murat Türkeş | Sinan Şahin
The study is aimed at investigating future changes in sustainability of olive farming by means of climate change, and changes in agricultural climatic suitableness and phenology of olive tree cultivation in the North Aegean sub-region of Türkiye mainly characterised with dry summer subtropical Mediterranean climate. According to projected changes based on RCP8.5 scenario, projected warming reaches an average of 5-6°C increase indicating most negative condition on olive cultivation. According to RCP8.5 scenario annual precipitation projections, present suitable areas of olive groves will decrease in the period of 2049-2073, and almost the entire study area will be in the category of medium suitable. With respect to projected Emberger Bioclimate classification, for RCP 8.5 scenario, there will be a significant increase in dry-sub humid areas in the period of 2049-2073. This increase will cover up the coastal areas in the period of 2074-2098, and even all the study region will be very likely characterised with dry-sub humid and semi-arid Mediterranean bioclimatic types except for some coastal areas. An increase of about 6°C is expected in maximum values of maximum air temperatures during the bud swelling periods in the spring, especially after 2050 under RCP8.5 scenario. This increase in extreme maximum temperatures may cause olive trees to bloom earlier and prolong the growth period. By regarding the high vernalization requirement of main olive variety in the study area, a 6°C temperature increase may significantly decrease olive yields and will force farmers to transition to new varieties with relatively low vernalization requirements. According to both RCP scenarios, there is a possibility of extension of suitable areas for olive cultivation towards low to mid-elevation plateaus and mid-elevation slopes of mountainous areas and high plateaus particularly facing suitable aspects to lower negative effects of projected future warming and dryness.
Show more [+] Less [-]DNA Barcoding and Phylogenetic Analysis of Culex and Anopheles Species in Siirt, Türkiye Full text
2025
Halil Dilmen | Behcet İnal | Mehmet Salih Özgökçe | Mustafa Cemal Çiftçi | Hilmi Kara | Sedriye Çatkın | Meryem Özer Dilmen | Gülcihan Koyunçu
Mosquitoes play a critical role as disease vectors, making them significant in terms of both public health concerns and ecological balance. This study aims to identify mosquito specimens collected from Siirt city center and six different districts using morphological and molecular methods. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region was used for molecular diagnosis. The findings revealed four mosquito species: Culex theileri Theobald, 1903, Culex mimeticus Noè, 1899, Culex quinquefasciatus Say, 1823, and Anopheles superpictus Grassi, 1899. Mitochondrial gene PCR products were sequenced, and the sequences were uploaded to the NCBI database for public access. Phylogenetic analysis was conducted using these sequences to investigate the genetic distances and evolutionary relationships among the mosquito species. In the phylogenetic analysis, Chironomus kiiensis was used as an outgroup. The analysis revealed that C. quinquefasciatus and A. superpictus were had the highest genetic distance (0.16), while the closest genetic distance was observed between C. quinquefasciatus and C. theileri (0.06). This study is presented as a preliminary investigation into the genetic diversity, evolutionary relationships, and population dynamics of mosquito species in Siirt Province. Further studies with a larger sample size and additional sequences are needed to establish more comprehensive phylogenetic relationships. The molecular findings contribute significantly to the systematic and ecological studies of mosquitoes.
Show more [+] Less [-]Boosting the Productivity of Bread Wheat (Triticum aestivum L.) Varieties through Optimal Seed Rates and Appropriate Systems for Irrigation Production System of Northwestern Ethiopia Full text
2025
Zelalem Yadelew
The availability of high-yielding varieties adapted to diverse agro-ecologies and production systems, preferred by farmers and consumers, is the key factor limiting productivity. Farmers access seeds of different quality levels from various seed systems. Studies on seed systems and rates in relation to yield and yield traits of bread wheat varieties under irrigation are limited. Therefore, an experiment was conducted in 2021 in Northwestern Ethiopia to improve the productivity of bread wheat (Triticum aestivum L.) varieties through suitable seed rates and systems under irrigation. The experiment was conducted using a randomized complete block design in a factorial arrangement of two varieties (Kakaba and Ogolcho), three seed systems (formal, intermediate and informal), and three seed rates (125, 150, and 175 kg ha-1) in three replications. SAS software was used for analysis. The results showed that the seed system and variety interaction had a significant effect (P < 0.05) on productive tiller numbers, days to 50% heading, kernel numbers per spike, and plant height. Additionally, the productive tiller numbers and days to 50% heading were significantly (P < 0.05) influenced by the interaction of variety with seed rate. However, the main effects of seed system, seed rate, and variety alone had a significant (P < 0.05) impact on physiological maturity, 1000-seed weight, kernel length, grain yield, biomass yield, and harvest index. The highest grain yields were obtained from the following main factors: seed system (intermediate (4.52 t ha-1); seed rate (150 kg ha-1 (4.71 t ha-1)); and variety (Kakaba (4.47 t ha-1)), which exceeded the average yield in irrigation (4.4 t ha-1). It is necessary to conduct experiments involving a greater number of seed rates and varieties over multiple cropping seasons and locations and sowing dates to strengthen the results.
Show more [+] Less [-]Trabzon Hurma Püresinin Reolojik Davranışının Modellenmesi Full text
2025
Sevim Gürdaş Mazlum | Dilan Lodos
Bu çalışmada Trabzon hurması (Diospyros kaki L.) püresinin akış davranışını en iyi tanımlayan reolojik modelin belirlenmesi amacıyla pürenin reolojik özellikleri çeşitli pH’larda (4,0, 5,5 ve 7,0), konsantrasyonlarda (%15, %17,5 ve %20), sıcaklıklarda (25, 50 ve 75°C) ve kayma hızında (8,4–28 s−1) araştırılmıştır. Deneysel veriler Power Law, Herschel-Bulkley, Casson ve Mizhari-Berk modellerine uygulanmıştır. Reolojik modellerin akış davranışını açıklamadaki uygunluğunu doğrulamak için belirleme katsayısı (R2), hata kareleri ortalamasının kare kökü (RMSE) ve indirgenmiş ki- kare (ꭓ2) olmak üzere üç istatistiksel ölçüt kullanılmıştır. Herschel-Bulkley ve Mizhari-Berk, hurma püresinin tüm işlem koşullarında deneysel verilere en iyi uyum sağlayan modeller olduğu görülmüştür. Ancak Mizhari-Berk modeli R2 ≥ 0.983, RMSE ≤ 0.0683 ve ꭓ2 ≤ 0.0160 istatistiksel parametre değerleriyle hurma püresinin akış davranışını en iyi tanımlayan model olmuştur. Trabzon hurması püresi psödoplastik (kayma incelmesi) ve Newtonyen olmayan bir davranış (n<1) sergilemiştir.
Show more [+] Less [-]Glutensiz Şekerpare Üretiminde Farklı Un Çeşitleri, Protein Kaynakları ve Transglutaminaz Enziminin Etkileri Full text
2025
Ruşen Metin Yıldırım
Şekerpare, Türkiye ve Orta Doğu mutfağının önemli tatlılarından biri olup, genellikle irmik kullanılarak hazırlanmaktadır. Ancak irmiğin gluten içermesi, çölyak hastalarının bu tatlıyı tüketmesini engellemektedir. Bu çalışmada, gluten içermeyen mısır unu, pirinç unu, patates unu, mısır nişastası ve tapyoka nişastası kombinasyonları kullanılarak glutensiz şekerpare formülasyonları geliştirilmiştir. Ayrıca, bu formülasyonlara eklenen soya proteini, bezelye proteini ve transglutaminaz (TG) enziminin şekerpare hamuru ve son ürün özellikleri üzerindeki etkileri incelenmiştir. Çalışma kapsamında, hamurların pH, sertlik, yapışkanlık, adhezyon işi ve hamur kuvveti gibi özellikleri değerlendirilirken son ürünlerde ise renk, sertlik, kırılganlık ve duyusal analizler gerçekleştirilmiştir. Elde edilen sonuçlar, kullanılan protein ve TG enziminin hamur özellikleri üzerindeki etkisinin kullanılan un kombinasyonuna bağlı olarak değiştiğini göstermiştir. Mısır ve patates unu ile hazırlanan hamurlar en yüksek sertlik değerine sahipken, tüm örneklerde kontrol grubu hamurları en düşük sertlikte olmuştur. Soya ve bezelye proteini, hamurun yapışkanlığını azaltırken, TG enziminin bezelye proteiniyle birlikte kullanımı yapışkanlık düzeyini önemli ölçüde artırmıştır. Duyusal analizde panelistler, %62,5 mısır unu ve %37,5 pirinç unundan oluşan MuPr reçetesiyle hazırlanan şekerpare örneklerini, hem yapı hem de lezzet açısından en beğenilen ürün olarak değerlendirmiştir. Bununla birlikte, kullanılan protein türü ve TG enzimi duyusal özellikler üzerinde genel anlamda önemli bir fark yaratmamıştır.
Show more [+] Less [-]Impact of Ultrasound-assisted Cooking and Endpoint Core Temperature on Physicochemical and Microbiological Properties, and Oxidative Stability of Beef Full text
2025
Dilara Aydın | Birol Kılıç | Azim Şimşek
This research aimed to investigate the impacts of different cooking methods (B; Boiling, US; Ultrasound-assisted slow boiling, UF; Ultrasound-assisted fast boiling) and endpoint core temperatures (ECT; 68°C, 74°C, and 80°C) on the oxidative stability, physicochemical, and microbiological properties of beef during refrigerated storage. The results demonstrated that UF application resulted in the lowest cooking loss (CL) at 74°C ECT. The US application caused a lower water activity (aw) compared to B. The lowest oxidation-reduction potential (ORP) levels were determined in UF, whereas the US had the highest ORP levels. Ultrasound-assisted cooking did not affect pH, yeast-mold and total mesophilic aerobic bacteria (TMAB) counts. On the other hand, UF and US caused an increase in total coliform counts compared to B. According to the results of lipid hydroperoxide (LPO) and thiobarbituric acid reactive substances (TBARS), UF application was more effective in preventing lipid oxidation compared to US and B. pH, CL, ORP, hue angle (hab) and b* values increased as the ECT increased, whereas aw, a*, chroma (C*ab) and browning index (BI; inner) values decreased. In addition, beef pieces cooked at 74°C or 80°C ECT had lower L* values, TMAB, and total coliform counts, and higher TBARS and LPO values than those cooked at 68°C ECT. 74°C was more effective in controlling microbiological changes, whereas 68°C was a better ECT for maintaining oxidative stability. In conclusion, UF has the potential to be an effective processing technology for improving oxidative stability and physicochemical properties of beef.
Show more [+] Less [-]Yalova Üniversitesi Öğrencilerinin Su Ürünleri Tüketim Alışkanlıklarının Değerlendirilmesi Full text
2025
Aydın Aytaç Gürdal | Selin Kayalı
Bu çalışma, Yalova Üniversitesi’nde eğitim gören öğrencilerin su ürünleri tüketim alışkanlıkları ve tercihlerini belirlemeyi amaçlamakta olup, çevrim içi bir anket yöntemiyle ve gönüllülük esasına dayanarak toplam 315 öğrenciyle gerçekleştirilmiştir. Katılımcıların %67’si kadın, %33’ü erkek olup, %88’i 18-25 yaş aralığındadır. Araştırma sonuçlarına göre, öğrencilerin %60’ı tavuk eti tüketmekte, balık eti tüketiminin ise %26 olduğu tespit edilmiştir. Öğrencilerin %29’u ayda bir kez balık tüketmekte, %54’ü ise tükettikleri balık miktarını yeterli bulmamaktadır. Katılımcıların %40’ı yetiştiriciliği yapılan su ürünlerini sağlıklı olarak değerlendirmekte, %16’sı ise su ürünleri yerine omega-3 gibi takviye edici gıdaların tercih edilebileceğini ifade etmektedir. Ayrıca, %40’ı su ürünlerini yeterince tüketmediklerini düşünmektedir. Öğrencilerin su ürünlerinin faydaları hakkında genel bilgi kaynakları arasında mobil cihazlar ve yakın çevrelerindeki insanlar öne çıkmaktadır. Balık satın alırken katılımcıların öncelikli olarak tazeliğe ve fiyata dikkat ettikleri, balığı genellikle balıkçılardan satın aldıkları belirlenmiştir. En çok tercih edilen balık türü deniz balıkları olup, katılımcıların büyük kısmı işlenmiş su ürünlerini tercih etmemektedir. Balık pişirme yöntemleri arasında ise en yaygın tercih kızartmadır. Balık dışında en çok tüketilen su ürünleri midye ve karides iken, su ürünleri tüketiminin en yoğun olduğu dönem ise balıkçılık sezonunun kapsadığı kış aylarıdır. Çalışma sonuçları, Yalova Üniversitesi öğrencilerinin su ürünleri tüketimini artırmaya yönelik çeşitli stratejilerin geliştirilmesi gerektiğini göstermektedir. Özellikle öğrenciler arasında sağlıklı beslenme bilincinin artırılması için su ürünlerinin faydaları konusunda daha etkili bilgilendirme çalışmaları yapılmalı ve su ürünlerinin taze, uygun fiyatlı ve ulaşılabilir olmasını sağlamak amacıyla yerel balıkçılık pazarları desteklenmelidir. Ayrıca, öğrenci barınma yerlerinde su ürünlerinin menülere dahil edilmesi, beslenme ve su ürünleri tüketimi konusunda eğitimlerin müfredata eklenmesi de bu tüketimi artırmaya yönelik önemli adımlar olacaktır.
Show more [+] Less [-]Combining Ability for Grain Yield and Popping Expansion in Popcorn (Zea mays everta) Hybrids Full text
2025
Oloruntoba O. Olakojo | Dotun J. Ogunniya | Adewole T. Akintunde | Samuel A. Olakojo
The poor yield and popping quality underscore the need for indigenous germplasm improvement. This study aimed to find parents with high general combining ability (GCA) and specific combining ability (SCA) for yield and popping traits. A Line × Tester design was used to hybridize 9 inbred lines and 4 testers, resulting in 36 hybrids. These hybrids were tested in field trials across three agro-ecologies of Nigeria in 2019 and 2020. Three replicates of 5 trials were set up in a Randomized Complete Block Design, and an air popper was used for popping tests. The study showed that crosses differed significantly at ρ≤0.01 for all agronomic traits except ears per plant (EPP) while the line × tester interaction was highly significant for all agronomic traits, except anthesis-silking interval and EPP. Parental lines V7, V9 and M3 had positive and significant GCA effect at ρ≤0.01 for grain yield, while V8, V9, and M3 were positive and significant at ρ≤0.01 for popping volume. Ten Hybrids had positive and significant SCA effect for grain yield and popping volume. Non-additive gene effects were more important in the inheritance of most agronomic studied traits, as evidenced by the line × tester interaction contributing a larger proportion (52 – 86%) to hybrid variance compared to the line effects (10.51 - 44.80%) and tester effects (0.20 to 15.24%). The study identified parental lines V7, V9, and M3 as good general combiners, whereas M1×V1, M1×V3, M1×V4, M1×V6, M1×V7, M3×V4, M3×V5, M4×V7, M4×V8 and M4×V9 were considered for their superior specific combining ability across various environments, particularly for grain yield and popping volume.
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