ABSTRACT This study aimed to determine the doses of maximum agronomic and economic efficiency as a function of different amounts of roostertree [Calotropis procera (Ait.) R.Br.] biomass added to the soil, that results in the maximum yield of green grains of cowpea in the semi-arid of Rio Grande do Norte state, Brazil. The experiment was conducted at the “Rafael Fernandes” Experimental Farm of the Universidade Federal Rural do Semi-Árido (UFERSA), Alagoinha, RN, from August to November 2013. In the experiment was used a randomized completely block design with 5 replicates. The treatments consisted of 20, 35, 50, and 65 t ha-1 (on a dry matter basis) of roostertree biomass added to the soil. The evaluated characteristics were: number of green pods per m2, productivity and dry mass of green pods, number of green grains per pod, weight of 100 green grains, and dry mass of green grains. The following economic indicators were determined: gross and net incomes, production operational costs, rate of return, and profit margin. The maximum agronomic efficiency of the yield of cowpea green grain was reached at the yield of 3.05 t ha-1, using 61.0 t ha-1 of roostertree biomass in the soil. The maximum economic efficiency yielded a net income of R$ 8,701.42, at the production of 3.02 t ha-1 green grains with 53.57 t ha-1 of roostertree biomass added to the soil. The use of roostertree as a green manure presents technical-economic feasibility in cowpea cultivation for green grains in the semi-arid conditions of Rio Grande do Norte. | RESUMO Este estudo teve como objetivo determinar as doses de eficiência agronômica e econômica máximas em função de diferentes quantidades de biomassa de flor-de-seda [Calotropis procera (Ait.) R.Br.] adicionadas ao solo, que resultem no rendimento máximo de grãos verdes de feijão caupi no semiárido do estado do Rio Grande Norte, Brasil. O experimento foi conduzido na Fazenda Experimental "Rafael Fernandes" da Universidade Federal Rural do Semi-Árido (UFERSA), Alagoinha, RN, no período de agosto a novembro de 2013. No experimento foi usado um delineamento de blocos completos casualizados com cinco repetições. Os tratamentos consistiram de 20, 35, 50 e 65 t ha-1 (em base seca) de biomassa de flor-de-seda adicionada ao solo. As características avaliadas foram: número de vagens verdes por m2, produtividade e massa seca de vagens verdes, número de grãos verdes por vagem, peso de 100 grãos verdes e massa seca de grãos verdes. Os seguintes indicadores econômicos foram determinados: rendas bruta e líquida, custos operacionais de produção, taxa de retorno e índice de lucratividade. A eficiência agronômica máxima do rendimento de grãos verdes de feijão-caupi foi alcançada com o rendimento de 3,05 t ha-1, utilizando-se 61,0 t ha-1 de biomassa de flor-de-seda incorporada ao solo. A eficiência econômica máxima produziu uma renda liquida de R$ 8.701,42, no rendimento de grãos verdes de 3.02 t ha-1 com 53,57 t ha-1 de biomassa de flor-de-seda adicionada ao solo. O uso da flor-de-seda como adubo verde apresenta viabilidade técnico-econômica no cultivo do feijão-caupi para grãos verdes nas condições semiáridas do Rio Grande do Norte.

ABSTRACT Intercropping is a cropping system that involves cultivating two or more crops in the same area with different cycles and vegetative architectures. One of the ways to increase productivity and efficiency in this cropping system is through green manuring and by properly considering crop combinations, cultivation timing, and planting configuration of the component crops. Thus, the aim of this study was to evaluate the productive performance of carrot and lettuce intercropping systems under different amounts of rooster tree biomass incorporated into the soil and under different spatial arrangements. The study was conducted at the experimental farm Rafael Fernandes, RN, Brazil, in the period from September 2012 to January 2013, in an experimental design of randomized complete blocks in a 4 x 3 factorial scheme, with twelve treatments and 3 replicates. The treatments consisted of the combination of four rooster tree biomass amounts incorporated into the soil (10, 25, 40 and 55 t ha-1 on a dry basis) with three predetermined spatial arrangements between the component crops (2:2, 3:3 and 4:4), which correspond to the rows of carrot alternating with the rows of lettuce. The agronomic performance of the carrot and lettuce intercropping system was optimized in the amount of approximately 46.36 t ha-1 of rooster tree incorporated into the soil. There was no influence of the spatial arrangements on the agronomic efficiency of the carrot and lettuce intercropping. The use of rooster tree as green manure is agronomically viable in intercropped systems of carrot and lettuce. | RESUMO A consorciação de culturas é um sistema de cultivo que envolve o cultivo de duas ou mais culturas na mesma área com diferentes ciclos e arquiteturas vegetativas. Uma das maneiras de aumentar a produtividade e a eficiência neste sistema de cultivo é através de adubação verde e considerando adequadamente as combinações de culturas, o tempo e a configuração de plantio das culturas componentes. Assim, o objetivo deste estudo foi avaliar o desempenho produtivo de sistemas consorciados de cenoura e alface em diferentes quantidades de biomassa de flor-de-seda incorporadas no solo e em diferentes arranjos espaciais. O estudo foi realizado na fazenda experimental Rafael Fernandes, RN, Brasil, no período de setembro de 2012 a janeiro de 2013, em um delineamento experimental de blocos completos casualizados, em esquema fatorial 4 x 3, com doze tratamentos e 3 repetições. Os tratamentos consistiram da combinação de quatro quantidades de biomassa de flor-de-seda incorporadas no solo (10, 25, 40 e 55 t ha-1 em base seca) com quatro arranjos espaciais predeterminados entre as culturas componentes (2:2, 3:3 e 4:4), que correspondem às fileiras de cenoura alternadas com as fileiras de alface. O desempenho agronômico do sistema de consórcio de cenoura e alface foi otimizado na quantidade de aproximadamente 46.36 t ha-1 de flor-de-seda incorporada no solo. Não houve influência dos arranjos espaciais sobre a eficiência agronômica do consórcio de cenoura e alface. O uso da flor-de-seda como adubo verde é agronomicamente viável em sistemas consorciados de cenoura e alface.

Brackish waters represent great potential for profitable agricultural production; however, productive usage depends on the adoption of proper cultural practices as well as a culture tolerant of salinity, which can require some restrictions related to soil and crop production. Given the lack of information pertaining to hydroponic melon culture, the objective of this study was to investigate physiological changes promoted by the use of brackish water in the preparation of the nutrient solution for melon (Cucumis melo L., cv. AF 015) growth in coconut fiber substrate under greenhouse conditions in Mossoró-RN, a semiarid region of Brazil. The experimental design was completely randomized, with 12 treatments arranged in a 4 x 3 factorial scheme, with 4 salt concentration levels (1.1 - control, 2.5, 4.0 and 5.5 dS m-1) and 3 exposition times (vegetative growth: 10-30 days after transplanting, DAT; flowering: 31 to 50 DAT; and fruiting and ripening: 51-70 DAT, which are the assessment phases of physiological maturation). Increasing salt concentrations in the nutrient solution reduced photosynthetic efficiency, stomatal conductance and transpiration, but increased the intercellular CO2 concentration in melon plants. A salt concentration in the low to intermediate range (2.5 dS m-1) resulted in the best water use efficiency by melon crops.

This study was conducted to evaluate the use of Curcuma longa (Linnaeus) extract in the in vitro control of Bipolaris oryzae (Breda de Haan) and to characterize the effect of this extract on rice seed germination. A completely randomized arranged in a factorial experimental design was used: three isolates of B. oryzae from rice seed from different rice-growing regions of Rio Grande do Sul (Fronteira Oeste, Campanha, and Sul) were tested with three concentrations (20, 40, and 80 mg/mL) of C. longa plus a control treatment (0 mg/mL). Each reaction was repeated in quadruplicate. The effect of the extract upon the disease development was evaluated based on mycelial growth (PMG) and spore production; rice seed germination was evaluated using a germination test (Germitest®). The PMG results demonstrate that the treatments were effective in reducing PMG, with a stronger response observed as the concentration of the extract increased. An average inhibition of 84% of sporulation was observed for the tested strains compared with the control treatment. There were, however, no significant differences in terms of seed germination test with the different C. longa concentrations. Therefore, treatment of rice seeds with C. longa extract does not affect seed germination but positively inhibits mycelial growth and sporulation, affecting the in vitro sporulation of the different isolates of B. oryzae.

This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

Low grain yield of cowpea is influenced by several production factors, especially inadequate soil fertilization, in particular, phosphorus application. Tropical soils have low phosphorus and zinc levels due to either the source material or the absence of fertilization, which restricts cowpea grain yield; besides of that, zinc deficiency is accentuated with phosphor application. This study aimed to evaluate the effect of fertilization with phosphorus and zinc on cowpea yield. Two experiments were conducted, one with ‘BRS Guariba’ cultivar and another with ‘BRS Aracê’ cultivar. Both of them were performed on a medium-texture dystrophic Yellow Latosol (Oxisol), in the city of Magalhães de Almeida, Maranhão state (Brazil), in 2015. The experimental design was a randomized complete block in a 4 x 4 factorial scheme, using four doses of phosphorus (0, 40, 80, and 120 kg P2O5 ha-1), and four of zinc (0, 2, 4, and 6 kg of Zn ha-1). There was an interaction between these minerals (p<0.01). Maximum grain yields of 1,376 kg ha-1 and 2,165 kg ha-1 were obtained at doses of 118 kg P2O5 ha-1 and 0.9 kg zinc ha-1 for ‘BRS Guariba’, and 120 kg P2O5 ha-1 and 3.1 kg zinc ha-1 for ‘BRS Aracê’, respectively. The production component of greatest influence on grain yield was the number of pods per plant, with average values of 6 and 10 for ‘BRS Guariba’ and ‘BRS Aracê’ cultivars, respectively. The mean correlation coefficient was 0.82 (p < 0.01).

Water deficit is a limiting factor for the soybean yield; it triggers different physiological and anatomical adaptations that have deleterious effects on the plants and can affect the selectivity of herbicides, causing production losses. In this context, the objective of this work was to evaluate the action of the chlorimuron-ethyl herbicide when applied at different stages of soybean plants, using conventional and transgenic cultivars, and different soil water potentials. A rate of 20 g ha-1 of the chlorimuron-ethyl herbicide was applied to two soybean cultivars (MG/BR46-Conquista - conventional, and BRS-Valiosa-RR - transgenic) at two phenological stages (V2 - first fully expanded trifoliate leaves, and V4 - third fully expanded trifoliate leaves), using three soil water potentials (-0.03 MPa, -0.07 MPa, and -0.5 MPa). Phytotoxicity, and plant height were evaluated at 3, 7, 14, and 21 days after the herbicide application. The shoot dry weight, root dry weight, and root system nodulation were evaluated. The soybean plants had lower phytotoxicity when subjected to application of chlorimuron-ethyl under water deficit conditions. The use of chlorimuron-ethyl reduced the growth and biomass of soybean plants and affected the plants' root system nodulation. The transgenic cultivar (BRS-Valiosa-RR) presented better performance when subjected to a moderate water deficit (-0.07 MPa), which contributes to biological nitrogen fixation.

Irrigation with saline water is a worldwide necessity an excess of salts in water or in soil causes growth inhibition, and negatively affects the productivity of many crops. Application of nitrogen fertilizers may be a way of mitigating the effects of salts on plants. The aim of this study was to evaluate the accumulation of biomass and the chlorophyll pigment content in jackfruit seedlings irrigated with water of increasing salinity level in soil with nitrogen sources. The treatments were distributed in randomized blocks with four replicates and three plants per plot, arranged in a 5 × 3 factorial scheme, related to electrical conductivity levels of the irrigation water of 0.3, 1.0, 2.0, 3.0 and 4.0 dS m-1, in soil without nitrogen, with ammonium sulfate and with urea, in a split application 60 and 75 days after sowing. An increase in the salinity of the water increased the salinity levels of soil, which was intensified by a dose of 150 mg of N, mainly when applied in the form of ammonium sulfate, inhibiting dry matter production and chlorophyll content in jackfruit seedlings. The greatest reductions in chlorophyll a and b content occurred in jackfruit seedlings irrigated with water of 4.0 dS m-1 conductivity in the soil without nitrogen fertilization. Urea is the most suitable nitrogen source for the production of seedlings under conditions of high salinity.

Vetiver grass is a member of the grass family Poaceae. Its fast development is probably due to the interaction with native microbiota, whose influence has not been studied yet. The objective of this work was to evaluate the colonization and development of the vetiver grass (Chrysopogon zizanioides (L.) Roberty) inoculated with arbuscular mycorrhizal fungi and dark septate endophytic fungi. The experimental design was a completely randomized design with six treatments (control, without mycorrhizal fungi, native inoculants, UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with three replicates each. Vetiver grass tillers as well as the native microbial inoculum were obtained from the Lower São Francisco river experimental area, located in Sergipe state, Northeastern Brazil. There was a negative interaction between all tested UFLAs mycorrhizal isolates and the native microbiota (mycorrhizal and endophytic fungi) in the treatments, especially when taking into consideration plant height and volume of roots. The effects of inoculation with UFLA isolates may have been influenced by the presence of the native mycorrhizal fungi and the dark septate endophytic fungi. Vetiver grass was responsive to the native inoculant. The mycorrhizal colonization of the vetiver grass was vesicular, but the formation of the arbuscules can be influenced by the interaction between the fungus, plant, and the environment.

This study aimed to determine the doses of maximum agronomic and economic efficiency as a function of different amounts of roostertree Calotropis procera (Ait.) R.Br. biomass added to the soil, that results in the maximum yield of green grains of cowpea in the semi-arid of Rio Grande do Norte state, Brazil. The experiment was conducted at the “Rafael Fernandes” Experimental Farm of the Universidade Federal Rural do Semi-Árido (UFERSA), Alagoinha, RN, from August to November 2013. In the experiment was used a randomized completely block design with 5 replicates. The treatments consisted of 20, 35, 50, and 65 t ha-1 (on a dry matter basis) of roostertree biomass added to the soil. The evaluated characteristics were: number of green pods per m2, productivity and dry mass of green pods, number of green grains per pod, weight of 100 green grains, and dry mass of green grains. The following economic indicators were determined: gross and net incomes, production operational costs, rate of return, and profit margin. The maximum agronomic efficiency of the yield of cowpea green grain was reached at the yield of 3.05 t ha-1, using 61.0 t ha-1 of roostertree biomass in the soil. The maximum economic efficiency yielded a net income of R$ 8,701.42, at the production of 3.02 t ha-1 green grains with 53.57 t ha-1 of roostertree biomass added to the soil. The use of roostertree as a green manure presents technical-economic feasibility in cowpea cultivation for green grains in the semi-arid conditions of Rio Grande do Norte.