RESUMO A consorciação de culturas é um sistema de produção capaz de reduzir os custos produtivos e aumentar a rentabilidade dos produtores de hortaliças, uma vez que ele possibilita o uso mais eficiente da área e dos insumos agrícolas. Com o objetivo de avaliar o efeito da densidade de plantas de alface na viabilidade econômica do consórcio de alface e rúcula, em duas épocas de cultivo (inverno e verão), dois experimentos foram instalados em campo, na Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Jaboticabal, São Paulo, Brasil. Os tratamentos corresponderam a cinco consórcios de alface com rúcula nos espaçamentos entre linhas da alface de: 0,20; 0,25; 0,30; 0,35 e 0,40 m, cinco cultivos solteiros de alface nos mesmos espaçamentos adotados em consórcio, e um cultivo solteiro de rúcula no espaçamento entre linhas de 0,20 m. O delineamento experimental utilizado foi em blocos casualizados completos, com quatro repetições. No inverno, maior rentabilidade é obtida com a maior densidade populacional. Maior rentabilidade das culturas, em ambas épocas de cultivo é obtida no verão. | ABSTRACT Intercropping is a production system that can reduce the production cost and increase the profitability of vegetable producers, since it permits more efficient land and agricultural inputs use. In order to evaluate the effect of lettuce plant density on the economic feasibility of lettuce-rocket intercropping system over two growing seasons (winter and summer), two experiments were conducted in the field at São Paulo State University “Júlio de Mesquita Filho” (UNESP), Jaboticabal, São Paulo, Brazil. The treatments consisted of five lettuce-rocket intercrops with spacing between lettuce rows of: 0.20; 0.25; 0.30; 0.35 and 0.40 m, five sole crop of lettuce in the same spacing between rows adopted in the intercropping system, and a sole cropping of rocket with spacing between rows of 0.20 m. The statistical design adopted was the complete randomized block with four replicates. During winter, higher profitability was achieved with the highest population density. Highest profitability of the crops in both seasons was obtained in summer.

Intercropping is a production system that can reduce the production cost and increase the profitability of vegetable producers, since it permits more efficient land and agricultural inputs use. In order to evaluate the effect of lettuce plant density on the economic feasibility of lettuce-rocket intercropping system over two growing seasons (winter and summer), two experiments were conducted in the field at São Paulo State University “Júlio de Mesquita Filho” (UNESP), Jaboticabal, São Paulo, Brazil. The treatments consisted of five lettuce-rocket intercrops with spacing between lettuce rows of: 0.20; 0.25; 0.30; 0.35 and 0.40 m, five sole crop of lettuce in the same spacing between rows adopted in the intercropping system, and a sole cropping of rocket with spacing between rows of 0.20 m. The statistical design adopted was the complete randomized block with four replicates. During winter, higher profitability was achieved with the highest population density. Highest profitability of the crops in both seasons was obtained in summer.

RESUMO Em frutos climatéricos, como é o caso da manga, as perdas vão da colheita ao consumo. Tendo como controle a refrigeração e a modificação da atmosfera passivamente, com emprego de revestimentos comestíveis e biodegradáveis ou o acondicionamento em filmes plásticos. Objetivou-se avaliar a influência da cera de carnaúba e embalagem de filme plástico Xtend, utilizando ou não refrigeração na conservação pós-colheita de manga ‘Tommy Atkins’. Os frutos foram revestidos com solução de cera de carnaúba e/ou acondicionados nas embalagens Xtend e armazenados nas temperaturas de 25 ± 3 ºC e 12 ± 1 ºC, por 21 dias. A cada sete dias foram realizadas avaliações quanto à perda de massa, firmeza do fruto, sólidos solúveis totais, acidez total titulável e vitamina C dos frutos e concentração de CO2 nas embalagens. Verificou-se que o armazenamento refrigerado retardou a velocidade das reações de degradação, auxiliando na conservação de mangas ‘Tommy Atkins’. O revestimento dos frutos com cera de carnaúba e embalagem Xtend, sob refrigeração, possibilitou o seu armazenamento por 21 dias a 12 ± 1 ºC, reduzindo a concentração de CO2, a perda de massa e a diminuição da acidez dos frutos e preservando a firmeza. | ABSTRACT For climacteric fruits, such as mango, losses have been reported from harvest to fruit consumption. Thus, controlled refrigeration and passive modified atmosphere have been used in combination with edible and biodegradable coatings or plastic packaging to minimise these losses. The present study aimed to evaluate the influence of carnauba wax and Xtend plastic packaging film, with and without refrigerated storage, on the post-harvest preservation of 'Tommy Atkins' mangoes. The fruits were coated with carnauba wax and/or wrapped in Xtend bags and then stored at 12 ± 1 and 25 ± 3 °C for 21 days. The weight loss, fruit firmness, total soluble solids, titratable acidity, vitamin C content, and the CO2 concentration inside the package were determined at 7-day intervals. Cold storage was observed to slow down the rate of degradation and to reduce adverse degradation reactions. By coating the fruits with carnauba wax, packaging them with Xtend plastic and keeping them under refrigeration, it was possible to store the mangoes at 12 ± 1 °C for 21 days. In this way, the CO2 concentration was reduced, mass loss and acidity were decreased, and the fruit firmness was preserved.

For climacteric fruits, such as mango, losses have been reported from harvest to fruit consumption. Thus, controlled refrigeration and passive modified atmosphere have been used in combination with edible and biodegradable coatings or plastic packaging to minimise these losses. The present study aimed to evaluate the influence of carnauba wax and Xtend plastic packaging film, with and without refrigerated storage, on the post-harvest preservation of 'Tommy Atkins' mangoes. The fruits were coated with carnauba wax and/or wrapped in Xtend bags and then stored at 12 ± 1 and 25 ± 3 °C for 21 days. The weight loss, fruit firmness, total soluble solids, titratable acidity, vitamin C content, and the CO2 concentration inside the package were determined at 7-day intervals. Cold storage was observed to slow down the rate of degradation and to reduce adverse degradation reactions. By coating the fruits with carnauba wax, packaging them with Xtend plastic and keeping them under refrigeration, it was possible to store the mangoes at 12 ± 1 °C for 21 days. In this way, the CO2 concentration was reduced, mass loss and acidity were decreased, and the fruit firmness was preserved.

RESUMO As altas taxas de luminosidade e elevadas temperaturas médias anuais da região semiárida do Brasil, associadas ao uso de irrigação e à adaptabilidade da cultura do girassol ao clima local podem favorecer o aumento da produtividade, devido à aceleração dos seus processos morfofisiológicos. Assim, objetivou-se avaliar o desempenho morfofisiológico e produtivo de variedades de girassol, sob cultivo irrigado por gotejamento, no Submédio São Francisco. O experimento foi desenvolvido no Campo Experimental da Embrapa Semiárido, em Petrolina, PE, em delineamento de blocos casualizados, sendo os tratamentos representados por 21 variedades de girassol, com quatro repetições. As variáveis analisadas foram: período de floração, altura, número de folhas, diâmetro do caule e do capítulo, número de plantas acamadas e quebradas, curvatura do caule, índice relativo de clorofila, fotossíntese, condutância estomática, transpiração, temperatura média foliar, peso de 1000 aquênios e produtividade das variedades de girassol. Verificou-se que o tempo de floração varia de 43 a 59 dias após o plantio, a altura das plantas de 1,0 a 1,4 m, o diâmetro dos capítulos de 0,154 a 0,221 m, o teor de clorofila de 30,8 a 33,98 e o peso de 1000 aquênios de 35,61 a 80,30 g nas variedades de girassol avaliadas. As variedades V7 e V8 se destacam por apresentarem produtividade acima de 2.960 kg de aquênios por hectare e baixo número de plantas acamadas e quebradas, indicando maior adaptabilidade ao cultivo irrigado na região do Submédio São Francisco. | ABSTRACT The high luminosity rates and high annual average temperatures of the Brazilian semiarid region, combined with the use of irrigation and adaptability of the sunflower crop to local climate, favor increased achene yield due to acceleration of morphophysiological processes. The objective of this work was to evaluate the morphophysiological and productive characteristics of sunflower varieties grown under drip irrigation in the Sub-Mid São Francisco River Valley. The experiment was conducted in the Experimental Field of the Brazilian Agricultural Research Corporation (Embrapa Semiarid), in Petrolina, State of Pernambuco, Brazil, in a randomized block design, with 21 sunflower varieties and four replications. The variables evaluated were flowering time, plant height, number of live leaves, stem and capitulum diameter, number of lodged and broken plants, stem curvature, relative chlorophyll index, photosynthesis, stomatal conductance, transpiration, average leaf temperature, 1000-achene weight and achene yield of the sunflower varieties. The treatments presented flowering time of 43 to 59 days after sowing, plant height of 1.0 to 1.4 m, capitulum diameter of 0.154 to 0.221 m, chlorophyll content of 30.8 to 33.98 Spad units and 1000-achene weight of 35.61 to 80.30 g. The sunflower varieties V7 and V8 stood out, with achene yields above 2,960 kg ha-1 and low number of lodged and broken plants, indicating a greater adaptability irrigation crops in the Sub-Mid São Francisco River Valley.

The high luminosity rates and high annual average temperatures of the Brazilian semiarid region, combined with the use of irrigation and adaptability of the sunflower crop to local climate, favor increased achene yield due to acceleration of morphophysiological processes. The objective of this work was to evaluate the morphophysiological and productive characteristics of sunflower varieties grown under drip irrigation in the Sub-Mid São Francisco River Valley. The experiment was conducted in the Experimental Field of the Brazilian Agricultural Research Corporation (Embrapa Semiarid), in Petrolina, State of Pernambuco, Brazil, in a randomized block design, with 21 sunflower varieties and four replications. The variables evaluated were flowering time, plant height, number of live leaves, stem and capitulum diameter, number of lodged and broken plants, stem curvature, relative chlorophyll index, photosynthesis, stomatal conductance, transpiration, average leaf temperature, 1000-achene weight and achene yield of the sunflower varieties. The treatments presented flowering time of 43 to 59 days after sowing, plant height of 1.0 to 1.4 m, capitulum diameter of 0.154 to 0.221 m, chlorophyll content of 30.8 to 33.98 Spad units and 1000-achene weight of 35.61 to 80.30 g. The sunflower varieties V7 and V8 stood out, with achene yields above 2,960 kg ha-1 and low number of lodged and broken plants, indicating a greater adaptability irrigation crops in the Sub-Mid São Francisco River Valley.

RESUMO O objetivo foi avaliar a influência das características morfológicas dos cladódios sobre a formação do dossel da palma forrageira. O estudo foi conduzido em Serra Talhada, PE, com os clones IPA Sertânia - IPA, Miúda - MIU e Orelha de Elefante Mexicana - OEM, os quais foram submetidos a três lâminas (2,5; 5,0; e 7,5 mm) e três intervalos de irrigação (7; 14 e 28 dias), no período compreendido entre março 2012 a agosto de 2013. Dados das dimensões dos cladódios e do dossel das plantas foram obtidos ao longo do tempo. A matriz de correlação de Pearson foi elaborada, e análises canônicas e de trilha foram aplicadas para avaliação da associação entre as variáveis. Verificou-se que, as características dos cladódios de 2ª e de 3ª ordens foram as que mais contribuíram na formação do dossel dos clones do gênero Nopalea (IPA Sertânia e Miúda), enquanto para a Orelha de Elefante Mexicana do gênero Opuntia foram as dos cladódios de primeira 1ª e 3ª ordens. Conclui-se que, a influência das variáveis dos cladódios sobre o crescimento do dossel da palma forrageira esteve mais associada às características do gênero, do que a peculiaridades dos clones. | ABSTRACT Here we aimed to evaluate the influence of cladode morphology on the canopy formation of forage cactus plants. The study was carried out in Serra Talhada, the State of Pernambuco, using the IPA Sertânia (IPA), Miúda (MIU), and Orelha de Elefante Mexicana (OEM) clones, which were submitted to three irrigation depths (2.5, 5.0, or 7.5 mm) and three irrigation intervals (7, 14, or 28 days) from March 2012 to August 2013. Cladode and plant canopy biometric data were obtained during the experimental period. We found that the characteristics of the second and third order cladodes contributed most to the canopy formation of clones of the genus Nopalea (IPA and MIU), whereas, for the genus Opuntia (OEM), the first and third order cladodes contributed most. Overall, we found that the influence of the cladode variables on the canopy growth of forage cactus is more associated with the characteristics of the genus than to the peculiarities of the clones.

Here we aimed to evaluate the influence of cladode morphology on the canopy formation of forage cactus plants. The study was carried out in Serra Talhada, the State of Pernambuco, using the IPA Sertânia (IPA), Miúda (MIU), and Orelha de Elefante Mexicana (OEM) clones, which were submitted to three irrigation depths (2.5, 5.0, or 7.5 mm) and three irrigation intervals (7, 14, or 28 days) from March 2012 to August 2013. Cladode and plant canopy biometric data were obtained during the experimental period. We found that the characteristics of the second and third order cladodes contributed most to the canopy formation of clones of the genus Nopalea (IPA and MIU), whereas, for the genus Opuntia (OEM), the first and third order cladodes contributed most. Overall, we found that the influence of the cladode variables on the canopy growth of forage cactus is more associated with the characteristics of the genus than to the peculiarities of the clones.

ABSTRACT The most-used preparation process of protein isolates (PI) involves the isoelectric precipitation of the protein. Heating shortens the preparation time but this procedure may affect the purity, yield, molecular profile of the protein, and the activity of the trypsin inhibitor. This study aimed to investigate the effect of heating in the production of cowpea protein isolates. Crude whole beans (WB) were defatted with hexane, and the protein isolates obtained by isoelectric precipitation with (HPI), and without (NHPI) heating. The protein content of the WB and the PI was determined by the micro-Kjeldahl method, and the extraction yield estimated from the protein content at the end of extraction in relation to this content in the raw material. Possible losses of protein fractions were followed by SDS-PAGE, and the trypsin inhibitor activity determined by an enzymatic assay (BAPNA: benzoyl-DL-arginine-p-nitroanilide). Protein content in HPI was 83.3%, less than in the NHPI (92.2%). The HPI yield was lower (40.0%) as compared to the NHPI (42.3%). Electrophoresis indicated bands ranging from 13 to 262 kDa in WB; and the NHPI presented a protein fraction’s profile closer to that of the WB than to the HPI. The WB had the trypsin inhibitor activity, expressed as Trypsin Inhibitory Units (TIU), of 32.5±0.5 TIU /mg-protein; HPI showed 12.7±0.5 TIU /mg-protein (39% of that observed in WB) and the NHPI, 8.3±0.2 TIU /mg-protein (25.5%). Heating reduces the yield and purity of proteins in the isolates. However, the inhibitory activity of trypsin cowpea is most affected by the isolation procedure. | RESUMO O processo mais utilizado na preparação de isolado proteico (IP) envolve a precipitação isoelétrica da proteína. O aquecimento abrevia o tempo de preparo dos isolados. Entretanto, esse procedimento pode afetar a pureza, rendimento, perfil molecular da proteína e presença do inibidor de tripsina. Este trabalho objetivou investigar o efeito do aquecimento na produção de IP de feijão-caupi. O feijão integral (FI) cru foi moído e desengordurado com hexano e o IP foi obtido por precipitação isoelétrica com aquecimento (IPCA) e sem aquecimento (IPSA). Determinou-se o teor proteico do FI, e dos isolados pelo método de micro-Kjeldahl. Estimou-se o rendimento da extração, pela massa de proteína ao final da extração em relação à existente inicialmente no material seco. Possíveis perdas de frações proteicas foram avaliadas por eletroforese SDS-PAGE. A atividade inibitória da tripsina foi avaliada em ensaio enzimático (BAPNA: benzoil-DLarginina-p-nitroanilida). Obteve-se um IPCA (83,3% de proteína), menos puro que o IPSA (92,2%). O rendimento do IPCA foi menor (40,0%) em relação ao IPSA (42,3%). A eletroforese indicou bandas variando de 13 a 262 kDa no FI, o IPSA apresentou um perfil de frações proteicas mais próximo ao FI que o IPCA. O FI apresentou atividade inibitória, expressa em Unidades Inibitórias de Tripsina (UIT) de 32,5±0,5UIT/mg-proteína, o IPCA apresentou 12,7±0,5UIT/mg-proteína (39% do observado no FI) e, o IPSA, 8,3±0,2 UIT/mg-proteína (25,5%). O aquecimento reduz o rendimento e a pureza da proteína nos isolados. Entretanto, a atividade inibitória da tripsina é mais afetada pelo processo de isolamento.

The most-used preparation process of protein isolates (PI) involves the isoelectric precipitation of the protein. Heating shortens the preparation time but this procedure may affect the purity, yield, molecular profile of the protein, and the activity of the trypsin inhibitor. This study aimed to investigate the effect of heating in the production of cowpea protein isolates. Crude whole beans (WB) were defatted with hexane, and the protein isolates obtained by isoelectric precipitation with (HPI), and without (NHPI) heating. The protein content of the WB and the PI was determined by the micro-Kjeldahl method, and the extraction yield estimated from the protein content at the end of extraction in relation to this content in the raw material. Possible losses of protein fractions were followed by SDS-PAGE, and the trypsin inhibitor activity determined by an enzymatic assay (BAPNA: benzoyl-DL-arginine-p-nitroanilide). Protein content in HPI was 83.3%, less than in the NHPI (92.2%). The HPI yield was lower (40.0%) as compared to the NHPI (42.3%). Electrophoresis indicated bands ranging from 13 to 262 kDa in WB; and the NHPI presented a protein fraction’s profile closer to that of the WB than to the HPI. The WB had the trypsin inhibitor activity, expressed as Trypsin Inhibitory Units (TIU), of 32.5±0.5 TIU /mg-protein; HPI showed 12.7±0.5 TIU /mg-protein (39% of that observed in WB) and the NHPI, 8.3±0.2 TIU /mg-protein (25.5%). Heating reduces the yield and purity of proteins in the isolates. However, the inhibitory activity of trypsin cowpea is most affected by the isolation procedure.

ABSTRACT Flour production through milling is an alternative for the cowpea processing, when the aim is to obtain a product with higher added value. The objective of this work was to determine the chemical composition, the granulometry, and evaluate the microbiological characteristics of whole-bean flours from five cowpea cultivars (BRS Cauamé, BRS Guariba, BRS Xiquexique, BRS Novaera and BRS Itaim). Cowpea beans were dried in a oven (Fanem, 320-SE) at 50 °C for 6 hours, milled in a cyclone-type rotor mill (Tecnal, TE-651/2) and, subsequently, in a pulverizer-type semi-industrial mill (Fritsch, Pulverisette 14) to obtain the flour. The flour centesimal composition, total energetic value, mineral content, granulometry, microbiological characteristics, and technological properties-flour water absorption and solubility in water-were analyzed. All analyzes were performed in triplicate and the results expressed in mean ± standard deviation. The means were compared by the Scott-Knott test (p ≤ 0.05). The flour centesimal composition presented (g per 100 g of flour): 7.13 to 10.33 of moisture, 2.06 to 3.65 of ashes, 1.53 g to 2.90 of lipids, 21.73 to 25.77 of proteins, and 58.08 to 64.02 of carbohydrate. The flours had on average 359.04 Kcal (100 g) and presented high iron, zinc, copper, manganese, phosphorus and magnesium contents. The whole-bean flours from the cultivars BRS Cauamé and BRS Xiquexique are sources of calcium. Regarding the microbiological characteristics, all flours are innocuous. The water absorption index were within 1.77 and 2.20 g g-1 and the water solubility index within 17 and 23%. All whole-bean flours had good nutritional, technological and microbiological characteristics, and thus potential for use in bakery products. | RESUMO A produção de farinha por meio da moagem é uma alternativa para o processamento do feijão-caupi, na busca de um produto com maior valor agregado. Objetivou-se determinar a composição química, a granulometria e avaliar microbiologicamente farinhas integrais de cinco cultivares (BRS Cauamé, BRS Guariba, BRS Xiquexique, BRS Novaera e BRS Itaim) comerciais de feijão-caupi. Para a obtenção das farinhas procedeu-se a secagem dos grãos em estufa (marca Fanem, modelo 320-SE) a 50 °C, por 6 horas; com posterior moagem, primeiramente em moinho de facas tipo ciclone (marca Tecnal, modelo TE-651/2) e posteriormente, em moinho semi-industrial tipo pulverizador (marca Fritsch, modelo Pulverisette 14). Analisaram-se a composição centesimal, o valor energético total, o conteúdo mineral, a granulometria, as características microbiológicas, e as tecnológicas quanto a absorção e solubilidade em água das farinhas. As análises foram realizadas em triplicata e os resultados expressos em média ± desvio padrão. As médias foram comparadas pelo teste de Scott-Knott (p≤0,05). Os valores encontrados para composição centesimal foram: umidade entre 7,13 a 10,33 g/100g; cinzas de 2,06 a 3,65 g/100g; lipídios variaram de 1,53 g a 2,90 g/100g; proteínas entre 21,73 a 25,77 g/100g e o conteúdo de carboidratos variou de 58,08 a 64,02 g/100g. As farinhas ofertaram em média 359,04 Kcal/100g e apresentaram elevado teor de ferro, zinco, cobre, manganês, fósforo e magnésio, sendo as das cultivares BRS Cauamé e BRS Xiquexique fontes de cálcio. Sob o aspecto microbiológico as farinhas apresentaram-se inócuas. Os valores de IAA variaram de 1,77 a 2,20 g/g e ISA de 17 a 23%. Concluiu-se que todas as farinhas apresentaram bom perfil nutritivo, tecnológico e microbiológico, apresentando, portanto potencial para utilização no desenvolvimento de produtos de panificação.

Flour production through milling is an alternative for the cowpea processing, when the aim is to obtain a product with higher added value. The objective of this work was to determine the chemical composition, the granulometry, and evaluate the microbiological characteristics of whole-bean flours from five cowpea cultivars (BRS Cauamé, BRS Guariba, BRS Xiquexique, BRS Novaera and BRS Itaim). Cowpea beans were dried in a oven (Fanem, 320-SE) at 50 °C for 6 hours, milled in a cyclone-type rotor mill (Tecnal, TE-651/2) and, subsequently, in a pulverizer-type semi-industrial mill (Fritsch, Pulverisette 14) to obtain the flour. The flour centesimal composition, total energetic value, mineral content, granulometry, microbiological characteristics, and technological properties-flour water absorption and solubility in water-were analyzed. All analyzes were performed in triplicate and the results expressed in mean ± standard deviation. The means were compared by the Scott-Knott test (p ≤ 0.05). The flour centesimal composition presented (g per 100 g of flour): 7.13 to 10.33 of moisture, 2.06 to 3.65 of ashes, 1.53 g to 2.90 of lipids, 21.73 to 25.77 of proteins, and 58.08 to 64.02 of carbohydrate. The flours had on average 359.04 Kcal (100 g) and presented high iron, zinc, copper, manganese, phosphorus and magnesium contents. The whole-bean flours from the cultivars BRS Cauamé and BRS Xiquexique are sources of calcium. Regarding the microbiological characteristics, all flours are innocuous. The water absorption index were within 1.77 and 2.20 g g-1 and the water solubility index within 17 and 23%. All whole-bean flours had good nutritional, technological and microbiological characteristics, and thus potential for use in bakery products.

ABSTRACT Two experiments were conducted to evaluate the relative effects of controlled atmosphere (CA) associated with 1-methylcyclopropene (1-MCP; 1.0 μL L−1), induction of mass loss (IML; 2%), and low ethylene (LE; <0.04 μL L−1 of C2H4) on the quality preservation of ‘Laetitia’ plums. In experiment 1 (2010), the treatments evaluated were cold storage (CS; 21.0 kPa O2 + <0.03 kPa CO2), CA1 (1 kPa O2 + 1 kPa CO2), CA1 + 1-MCP, CA1 + IML, and CA1 + LE. In experiment 2 (2011), the treatments evaluated were CS, CA2 (2 kPa O2 + 2 kPa CO2), CA2 + IML, and CA2 + 1-MCP. In both experiments, the fruit were stored at 0.5 ± 0.1°C and 96 ± 2% RH. CA storage delayed fruit ripening in both atmosphere conditions evaluated and reduced the internal browning of the ‘Laetitia’ plums, particularly in CA2. 1-MCP, LE, and IML had additional effects to CA1 on preserving flesh consistency. 1-MCP, irrespective of the CA condition, and IML, in CA1, reduced internal browning. CA1, regardless of the complementary technologies, reduced the incidence of decay and fruit cracking. | RESUMO Foram conduzidos dois experimentos com o objetivo de avaliar o efeito da atmosfera controlada (AC) associada ao 1-metilciclopropeno (1-MCP; 1,0 μL L-1), à indução de perda de massa (IPM; 2%) e ao baixo etileno (BE; <0,04 μL L-1 de C2H4) sobre a manutenção da qualidade de ameixas ‘Laetitia’. No experimento 1, em 2010, os tratamentos avaliados foram armazenamento refrigerado (AR; 21 kPa O2 + <0,03 kPa CO2), AC1 (1 kPa O2 + 1 kPa CO2), AC1+1-MCP, AC1+IPM e AC1+BE. No experimento 2, em 2011, os tratamentos foram AR, AC2 (2 kPa O2 + 2 kPa CO2), AC2+1-MCP e AC2+IPM. Em ambos os experimentos, os frutos foram armazenados a 0,5±0,1 ºC e 96±2% de umidade relativa. A AC retardou o amadurecimento, em ambas as atmosferas avaliadas, e reduziu o escurecimento de polpa de ameixas ‘Laetitia’, especialmente na AC2. O 1-MCP, o BE e a IPM apresentaram efeito adicional à AC1 na manutenção da consistência da polpa. O 1-MCP, independente da condição de AC, e a IPM, na condição de AC1, reduziram o escurecimento da polpa. A AC1, independente do uso de tecnologias complementares, reduziu a incidência de podridões e de rachaduras.

Two experiments were conducted to evaluate the relative effects of controlled atmosphere (CA) associated with 1-methylcyclopropene (1-MCP; 1.0 μL L−1), induction of mass loss (IML; 2%), and low ethylene (LE; <0.04 μL L−1 of C2H4) on the quality preservation of ‘Laetitia’ plums. In experiment 1 (2010), the treatments evaluated were cold storage (CS; 21.0 kPa O2 + <0.03 kPa CO2), CA1 (1 kPa O2 + 1 kPa CO2), CA1 + 1-MCP, CA1 + IML, and CA1 + LE. In experiment 2 (2011), the treatments evaluated were CS, CA2 (2 kPa O2 + 2 kPa CO2), CA2 + IML, and CA2 + 1-MCP. In both experiments, the fruit were stored at 0.5 ± 0.1°C and 96 ± 2% RH. CA storage delayed fruit ripening in both atmosphere conditions evaluated and reduced the internal browning of the ‘Laetitia’ plums, particularly in CA2. 1-MCP, LE, and IML had additional effects to CA1 on preserving flesh consistency. 1-MCP, irrespective of the CA condition, and IML, in CA1, reduced internal browning. CA1, regardless of the complementary technologies, reduced the incidence of decay and fruit cracking.

ABSTRACT Knowledge of the nutrient uptake by crops at different stages of growth may provide the basis for rational application of fertilizers, which may lead to reduced production costs and improved use of fertilizers by plants. Based on this knowledge, we conducted this study to determine the biomass and nutrient accumulation curve of the onion cultivar Bella Vista in a system of transplanted seedlings. The experiment was carried out in Ituporanga-SC, Brazil, by using a randomized block design with three replicates. Eleven plant collections were carried out throughout the crop cycle. The variables evaluated included plant length; total dry biomass of leaves, bulbs, and roots; and concentrations of macro- and micronutrients. The maximum dry biomass accumulation in the shoots and bulbs at 140 days after transplanting (DAT) was 4.26 and 27.41 mg per plant, respectively, which contributed to 13% and 85% of the whole plant dry biomass at the end of the cycle, respectively. Potassium (K, 521.6 mg) was accumulated in the largest quantity per plant, followed by nitrogen (N, 465.7 mg), calcium (Ca, 253.3 mg), sulfur (S, 109.0 mg), magnesium (Mg, 86.9 mg), and phosphorus (P, 76.5 mg). The micronutrient accumulation was as follows: iron (Fe, 6,227.5 µg), boron (B, 902.3 µg), zinc (Zn, 573.7 µg), manganese (Mn, 573.7 µg), and copper (Cu, 241.4 µg). | RESUMO O conhecimento da extração de nutrientes pelas culturas, em diferentes estádios, pode fornecer subsídios para aplicação racional de fertilizantes, resultando na redução dos custos de produção e melhor aproveitamento dos fertilizantes pelas plantas. Desta forma, objetivou-se com este trabalho determinar a curva de acúmulo de fitomassa e nutrientes para a cebola cv. Bella Vista, em sistema de mudas transplantadas. O experimento foi instalado e conduzido no município de Ituporanga - SC, Brasil, utilizando o delineamento experimental utilizado foi em bloco ao acaso com três repetições. Foram avaliados o comprimento da planta, fitomassa seca total, das folhas, do bulbo e raízes e concentração de macro e micronutrientes. A máxima fitomassa seca de da parte aérea e bulbos aos 140 dias após o transplante foi de 4,26 e 27,41 mg por planta, respectivamente, o que contribuiu para 13% e 85% da massa seca total da planta no final do ciclo, respectivamente. O potássio (521,6 mg) foi acumulado em maior quantidade pela planta, seguido pelo nitrogênio (N, 465,7 mg), cálcio (Ca, 253,3 mg), ensofre (S, 109,0 mg), magnésio (Mg, 86,9 mg) e fósforo (P, 76,5 mg). Para os micronutrientes a ordem de acúmulo foi: ferro (Fe, 6.227,5 µg), boro (B, 902,3 µg), zinco (Zn, 573,7 µg), manganês (Mn, 573,7 µg), e cobre (Cu, 241,4 µg).

Knowledge of the nutrient uptake by crops at different stages of growth may provide the basis for rational application of fertilizers, which may lead to reduced production costs and improved use of fertilizers by plants. Based on this knowledge, we conducted this study to determine the biomass and nutrient accumulation curve of the onion cultivar Bella Vista in a system of transplanted seedlings. The experiment was carried out in Ituporanga-SC, Brazil, by using a randomized block design with three replicates. Eleven plant collections were carried out throughout the crop cycle. The variables evaluated included plant length; total dry biomass of leaves, bulbs, and roots; and concentrations of macro- and micronutrients. The maximum dry biomass accumulation in the shoots and bulbs at 140 days after transplanting (DAT) was 4.26 and 27.41 mg per plant, respectively, which contributed to 13% and 85% of the whole plant dry biomass at the end of the cycle, respectively. Potassium (K, 521.6 mg) was accumulated in the largest quantity per plant, followed by nitrogen (N, 465.7 mg), calcium (Ca, 253.3 mg), sulfur (S, 109.0 mg), magnesium (Mg, 86.9 mg), and phosphorus (P, 76.5 mg). The micronutrient accumulation was as follows: iron (Fe, 6,227.5 µg), boron (B, 902.3 µg), zinc (Zn, 573.7 µg), manganese (Mn, 573.7 µg), and copper (Cu, 241.4 µg).

ABSTRACT No-tillage cotton systems require soil coverage with cover crop residue for a longer time due to the late cycle of cotton. However, decomposition rates may vary between cover crops, and the adjustment of models to describe it is critical to no-tillage cotton management. Two non-linear regression models, exponential (EM) and Michaelis-Menten (MM), were adjusted to dry matter decomposition of cover crops in a cotton no-tillage system, in Brazil. Three field trials were performed in 2012 for the cover crops Urochloa ruziziensis (brachiaria), Pennisetum glaucum (pearl millet), and Cajanus cajan (pigeon pea). Samples of cover crop were collected at 20, 50, 70, 110, 140, and 170 days after sowing upland cotton to measure dry matter decomposition. MM showed better adjustment than EM for all cover crops. The estimations of half-life parameters were different between the cover crops, suggesting that each cover crop has its own rate of decomposition. For pearl millet, brachiaria, and pigeon pea, the half-life estimation by exponential model was over the MM in 9, 12, and 12 days. | RESUMO Para a semeadura direta é preciso adequada quantidade de palha sobre o solo, e devido ao longo ciclo do algodoeiro, sua persistência deve ser alta. Entretanto, como a decomposição dos resíduos vegetais ao longo do tempo pode variar entre as plantas de cobertura, o ajuste de modelos que melhor a descreva é essencial. Dois modelos de regressão não-linear, exponencial (ME) e Michaelis-Menten (MM), foram ajustados à decomposição de matéria seca de plantas de cobertura no sistema de semeadura direta do algodoeiro, em condições do Cerrado goiano. Três experimentos foram instalados no ano de 2012, cada um consistido de uma espécie de planta de cobertura, sendo Urochloa ruziziensis (braquiária ruziziensis), Pennisetum glaucum (milheto) e Cajanus cajan (guandu). Em cada ensaio, procedeu-se a amostragem da palha da planta de cobertura aos 20, 50, 70, 110, 140 e 170 dias após a semeadura do algodoeiro, para determinação da matéria seca remanescente. Para as três plantas de cobertura o modelo MM apresentou melhor ajuste que o ME. As estimativas dos tempos de meia-vida das palhadas via MM diferiram entre as plantas de cobertura, indicando que cada uma possui velocidade de decomposição específica. Para o milheto, o guandu e a braquiária, as estimativas de tempo de meia-vida pelo ME foram superiores às do MM em 9, 12 e 12 dias, respectivamente.

No-tillage cotton systems require soil coverage with cover crop residue for a longer time due to the late cycle of cotton. However, decomposition rates may vary between cover crops, and the adjustment of models to describe it is critical to no-tillage cotton management. Two non-linear regression models, exponential (EM) and Michaelis-Menten (MM), were adjusted to dry matter decomposition of cover crops in a cotton no-tillage system, in Brazil. Three field trials were performed in 2012 for the cover crops Urochloa ruziziensis (brachiaria), Pennisetum glaucum (pearl millet), and Cajanus cajan (pigeon pea). Samples of cover crop were collected at 20, 50, 70, 110, 140, and 170 days after sowing upland cotton to measure dry matter decomposition. MM showed better adjustment than EM for all cover crops. The estimations of half-life parameters were different between the cover crops, suggesting that each cover crop has its own rate of decomposition. For pearl millet, brachiaria, and pigeon pea, the half-life estimation by exponential model was over the MM in 9, 12, and 12 days.

RESUMO A mandioca é, geralmente, plantada em solos arenosos e de baixa fertilidade, tornando eminente a necessidade da mesma em realizar associações benéficas com micro-organismos que possam auxiliar seu crescimento. Nessa ótica, é evidente a possibilidade de seleção de isolados bacterianos eficientes em promover o crescimento da cultura, os quais podem fornecer subsídios para futuros inoculantes. O objetivo deste trabalho foi isolar, identificar, selecionar e avaliar a diversidade genética de bactérias endofíticas de raízes e caules de mandioca, cultivada em Garanhuns - PE, com características envolvidas na promoção de crescimento vegetal. O isolamento foi realizado em meio de cultura semissólido LGI-P. Os isolados selecionados foram avaliados quanto ao potencial de fixar N2, quanto à capacidade de produzir ácido indol acético, quanto à capacidade de solubilizar fosfato inorgânico e produzir exopolissacarídeos. Alguns isolados bacterianos tiveram o gene 16S rRNA sequenciados pelo método de Sanger. No total, foram obtidos 52 isolados de bactérias endofíticas de mandioca. Em relação ao potencial para fixar N2, 15% dos isolados foram positivos. Quanto à produção de AIA, 78% dos isolados produziram este fitohormônio em meio com o acréscimo de L-triptofano. Cerca de 31% dos isolados foram capazes de solubilizar fosfato inorgânico e 60% apresentaram produção de exopolissacarídeos. A identificação de 19 isolados possibilitou o agrupamento em seis gêneros bacterianos, sendo eles: Achromobacter, Bacillus, Burkholderia, Enterobacter, Pantoea e Pseudomonas. Plantas de mandioca cultivadas em Garanhuns - PE apresentam interação com diferentes grupos de bactérias endofíticas e há grupos bacterianos com diversas características envolvidas na promoção de crescimento vegetal. | ABSTRACT Cassava is mostly planted in sandy soils which are usually of low fertility, thereby making it necessary to perform beneficial associations with microorganisms that can promote their growth. In this perspective, the possibility of selecting bacterial isolates efficient in promoting the growth of the culture is evident, which can provide subsidies for future inoculants. The objective of this study was to isolate, identify, select and evaluate the genetic diversity of endophytic bacteria in roots and stems of cassava grown in Garanhuns - PE, with features involved in promoting plant growth. The isolation was performed on culture medium semisolid LGI-P. The selected isolates were evaluated for the potential to fix N2, as the ability to produce indole acetic acid, for their ability to solubilize inorganic phosphate and produce exopolysaccharides. Some bacterial isolates had their 16S rRNA gene sequenced by the Sanger method. A total of 52 endophytic bacteria isolates were obtained from cassava. Regarding the potential to fix N2, 15% of the isolates were positive. As for the production of IAA, 78% of the isolates produced this phytohormone in a medium with increased L-tryptophan. Approximately 31% of the isolates were able to solubilize inorganic phosphate and 60% had exopolysaccharide. The identification of 19 isolates allowed the grouping into six bacterial genera, namely: Achromobacter, Bacillus, Burkholderia, Enterobacter, Pantoea and Pseudomonas. Cassava plants grown in Garanhuns - PE present interaction with different groups of endophytic bacteria and there are bacterial groups with several characteristics involved in promoting plant growth.

Cassava is mostly planted in sandy soils which are usually of low fertility, thereby making it necessary to perform beneficial associations with microorganisms that can promote their growth. In this perspective, the possibility of selecting bacterial isolates efficient in promoting the growth of the culture is evident, which can provide subsidies for future inoculants. The objective of this study was to isolate, identify, select and evaluate the genetic diversity of endophytic bacteria in roots and stems of cassava grown in Garanhuns - PE, with features involved in promoting plant growth. The isolation was performed on culture medium semisolid LGI-P. The selected isolates were evaluated for the potential to fix N2, as the ability to produce indole acetic acid, for their ability to solubilize inorganic phosphate and produce exopolysaccharides. Some bacterial isolates had their 16S rRNA gene sequenced by the Sanger method. A total of 52 endophytic bacteria isolates were obtained from cassava. Regarding the potential to fix N2, 15% of the isolates were positive. As for the production of IAA, 78% of the isolates produced this phytohormone in a medium with increased L-tryptophan. Approximately 31% of the isolates were able to solubilize inorganic phosphate and 60% had exopolysaccharide. The identification of 19 isolates allowed the grouping into six bacterial genera, namely: Achromobacter, Bacillus, Burkholderia, Enterobacter, Pantoea and Pseudomonas. Cassava plants grown in Garanhuns - PE present interaction with different groups of endophytic bacteria and there are bacterial groups with several characteristics involved in promoting plant growth.