Cryopreservation of sheep red blood cells, 2: Purified polyvinylpyrrolidone and hydrolyzed starch as protective agents [preservation, low temperature, PVP, starch, cryoprotective agents]
1979
Myhrvold, V. (Oslo Univ. (Norway). Dept. of Microbiology)
Cryopreservation of sheep red blood cells. 2. Purified polyvinylpyrrolidone and hydrolyzed starch as protective agents. Acta vet. scand. 1979, 20, 531-536. - The protection of sheep erythrocytes against damage at low temperature (-196 deg. C) was investigated using purified polyvinylpyrrolidone (PVP) and hydrolyzed starch as cryoprotective agents. Identical results were obtained with untreated PVP, neutralized PVP and PVP purified by chromatography. With hydrolyzed starch the cryoprotection was dependent on the type and concentration of the starch used and on the extent of hydrolysis of the starch prior to use. Cell recovery with some starch types was the same (80-85 per cent) as that obtained with PVP.
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