The nitrate reductase stabilizing factor in cotton seeds [Gossypium hirsutum, Phaseolus vulgaris, bean, Citrullus vulgaris, water melon]
1980
Purvis, A.C. | Tischler, C.R. | Funkhouser, E.A. (Texas Agricultural and Mechanical Univ. (USA). Dept. of Plant Sciences)
A heat-stable factor present in dry cotton (Gossypium hirsutum L.) seed, or in cotyledons until day 4 of germination, is capable of stabilizing labile nitrate reductase from other species. The stabilizing factor has no effect on stability of glyceraldehyde-3-phosphate dehydrogenase, but slightly improves the stability of glucose-6-phosphate dehydrogenase. Treatment with protease III, and to a lesser extent, trypsin, reduces the effectiveness of the stabilizer. The stabilizer is not a trypsin inhibitor. Dialysis demonstrates that the stabilizing factor has a molecular weight greater than 12,000 daltons. The factor precipitates hetween 25 and 75 per cent (NH(,4))(,2)SO(,4) saturation, and is effective at protein concentrations much lower than those required when casein is employed. From the results of this study, we conclude that the factor which stabilizes labile nitrate reductase from cotton seed in proteinaceous
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