Effects of temperature on germination and mitochondrial dehydrogenases in two soybean (Glycine max) cultivars [glutamate dehydrogenase, isocitrate dehydrogenase, MDH, malate dehydrogenase (E.C. 1.1.1.37); GDH, glutamate dehydrogenase (E.C. 1.4.1.3); NADP-ICDH, NADP-linked isocitrate dehydrogenase (E.C. 1.1.1.42); S-1, mitochondrial intermembrane region; S-2, mitochondrial matrix space; ETP, mitochondrial cristal membrane fragments; P, probability]
1980
Henson, C.A. | Schrader, L.E. | Duke, S.H. (Wisconsin Univ. (USA). Dept. of Agronomy)
The effects of eight germination temperatures from 10 deg C to 35 deg C on germination and dehydrogenase activities of two soybean (Glycine max [L.] Merr.) cultivars were investigated after 48 h of seedling growth. Axis fresh weights of cv. Chippewa increased as germination temperature increased from 10 deg C to 35 deg C. In contrast, axis fresh weights for the cv. Wells increased more slowly with increasing temperature and reached a maximum at c. 25 deg C. In general, in vitro activities of glutamate dehydrogenase (GDH), NADP-isocitrate dehydrogenase (NADP-ICDH), and malate dehydrogenase (MDH) from the axes of cv. Chippewa correlated well with increases in axis fresh weights. GDH and MDH activities from axes of the cv. Wells also reflected increases in axis fresh weights although the correlation was not as evident as for the cv. Chippewa. NADP-ICDH activity from "Wells" axes was highest at 35 deg C even though germination was poor at this temperature. GDH and MDH activities from cotyledons of both cultivars were not correlated with axis weight increases. No GDH activity was detected in "Wells" cotyledons from seeds germinated at 35 deg C.
Mostrar más [+] Menos [-]Información bibliográfica
Este registro bibliográfico ha sido proporcionado por European Union
