Reassociation of cytoplasmic ribosomal subunits of barley and its virescens mutant to form active hybrids [wild type, ribosomes, polyphenylalanine synthesis, subunit reassociation]
1980
Koundal, K.R. | Cohen, A.S. | Zalik, S. (Alberta Univ. (Canada). Dept. of Plant Science)
Polyphenylalanine synthesis by cytoplasmic ribosomes of Gateway barley (Hordeum vulgare) and its virescens single gene nuclear mutant was compared. The cytoplasmic 80S ribosomes were isolated from unimbibed embryo material and the ribosomes were dissociated into their component 60S and 40S subunits by centrifugation through sucrose gradients containing high KCl-to-MgCl(,2) buffer. These separated subunits could be reassociated by resuspension in buffer having about equimolar concentrations of MgCl(,2) and KCl. Both homologous and heterologous combinations of the subunits reassociated to give monomeric 80S ribosomes, and the derived monomers as well as various combinations of the individual subunits showed equivalent activity in an in vitro system for poly (U)-directed polyphenylalanine synthesis
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