The effects of nitrogen sources on the expression of nif gene in Klebsiella pneumoniae nif-lac fusants
1985
Kim, S.H. | Son, H.J. | Kim, C.J. | Mheen, T.I. (Korea Advanced Inst. of Science and Technology, Seoul (Korea R.). Dept. of Microbiology Laboratory)
The effects of various nitrogen sources on the expression of nif gene were investigated using nif-lac fusants of Klebsiella pneumoniae. K. pneumoniae UN 2979 was infected with Mudl lysate prepared by heat induction of K. pneumoniae UN 4482. About 80 nif-lac fusants were isolated and designated as LX series. In the presence of NH4+, beta-galactosidase activities on nif-lac fusants were greatly repressed. Amino acids, such as serine, glutamine and asparagine, were found to support the growth of K. pneumoniae M5al quite well, and showed a repressive effect on beta-galactosidase activities of nif-lac fusants LX-9 and LX-22 in NFHM
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