Correlation between the quantity of haemolysin produced by Listeria monocytogenes strains and the virulence determined in mice
1989
Bernath, S. | Szemeredine Pitron, M. (Allatgyogyaszati Oltoanyag- es Gyogyszervizsgalo Intezet, Budapest (Hungary))
Correlation between the quantity of haemolysin of 12 L. monocytogenes strains with different virulence grades and mouse virulence was investigated. The quantity of haemolysin was determined in a system using Cr51 marked ovine erythrocytes. The activity was expressed in the germ counts of bacterial suspensions showing a 50 per cent haemolysis. Mouse virulence of strains was calculated by the method of Reed and Muench, following intaperitoneal inoculations. Based on the correlation calculations of the results of repeated investigations in case of 9 strains, numerical correlation could not be found between the quantity of haemolysin produced by the Listeria strains and mouse virulence under the applied experimental conditions. It has been pointed out that the quantity of haemolysin does not play a decisive role in the mechanism of virulence of Listeria strains.
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