Comparison on serological reaction between complement fixation test and enzyme-linked immunosorbent assay for detection of antibodies against sendai virus, mouse hepatitis virus and mycoplasma pulmonis in mice and rats

Chung, Y.Y. . Dept. of Agriculture; Lee, H.C.; Lee, E.; Yoo, B.S. . Coll. of Agriculture and Animal Science

Comparison on serological reaction between complement fixation test and enzyme-linked immunosorbent assay for detection of antibodies against sendai virus, mouse hepatitis virus and mycoplasma pulmonis in mice and rats

1989

Chung, Y.Y. (Korea Air and Correspondence Univ., Seoul (Korea R.). Dept. of Agriculture) | Lee, H.C. | Lee, E. | Yoo, B.S. (Youngnam Univ., Kyongsan (Korea R.). Coll. of Agriculture and Animal Science)

This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine (mice and rats) antibodies against hemagglutinating virus of Japan (HVJ), mouse hepatitis virus (MHV) and Mycoplasma pulmonis (Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immuno-sorbent assay (ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats

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