Identification, characterization, and utilization of sources of resistance to barley yellow dwarf virus
1990
Larkin, P.J. | Brettell, R.I.S. | Banks, P. | Appels, R. | Waterhouse, P.M. (Commonwealth Scientific and Industrial Organization, Canberra (Australia)) | Cheng, Z.M. | Zhou, G.H. | Xin, Z.Y. | Chen, X. (Chinese Academy of Agricultural Sciences, Beijing (China))
The enzyme-linked immunosorbent assay (ELISA) technique was used with a local isolate of the PAV serotype to screen wild grasses for resistance to barley yellow dwarf virus (BYDV). Thirteen species of grass showed resistance in that they could maintain low levels of the virus for one month after inoculation. The F1 hybrids with wheat (Triticum aestivum L.) showed good resistance. Particularly promising was a partial amphiploid from China called Zhong 4, which showed very low levels of PAV in ELISA. Probes with various ribosomal spacer sequences and 5S ribosomal sequences of DNA isolated from Zhong 4 suggested that some alien chromosomes present are closely related to the E genome. The partial amphiploid TAF 46 from Y. Cauderon and the derived single chromosome addition line, L1, showed good resistance to virus multiplication (PAV and RPV-like). We are attempting to introgress these resistances to wheat using cell culture induced non-homologous exchange or the ph effect.
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