Differential responses of N-nitrosoamines and aromatic amines in the plant cell/microbe coincubation assay
1993
Gichner, T. (Akademie Ved Ceske Republiky, Prague (Czech Republic). Ustav Experimentalni Botaniky) | Wagner, E.D. | Plewa, M.J.
The plant cell/microbe coincubation assay is based on employing living tobacco cells in suspension culture as the activating system for promutagens and the Ames/Salmonella cells as the genetic indicator system. In contrast to aromatic amines (e.g. 2-aminofluorene and m-phenylenediamine) that were previously reported to be activated to products mutagenic in the S. typhimurium strains TA 98 or YG1024 by tobacco cells, promutagenic N-nitrosoamines (N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosopiperidine, N-nitrosomethyl-2- hydroxypropylamine) were not activated to product(s) mutagenic in S. typhimurium TA 100.
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