Synchronous cell division in rice [Oryza sativa] suspension cultures and cell cycle specific expression of histone H3 and PCNA [Poliferating-Cell Nuclear Antigen] genes
1993
Nakasone, S. (National Inst. of Agrobiological Resources, Tsukuba, Ibaraki (Japan)) | Minami, E. | Imai, T. | Akiyama, F. | Ohashi, Y.
Two friable cell lines of rice suspension cultures with homogeneous cell population were selected. A cell division synchrony was induced with aphidicolin, a specific inhibitor of DNA polymerase-alpha. The number of cells doubled at each cell division. The mitotic index of the first division was about 13% the cell population. S-phase specific expression of histone H3 and PCNA (proliferating-cell nuclear antigen) genes was detected in this system
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