Effect of pretreatment and age of explants on calloid initiation of coconut inflorescence
1993
Orense, O.D. | Cueto, C.A. | Areza, M.B.B. | Rillo, E.P. | Ebert, A.W. (Philippine Coconut Authority, Banao, Guinobatan, Albay (Philippines). Philippine-German Coconut Tissue Culture Project)
Browning of explants and calloid initiation on immature coconut inflorescences of various ages collected from Catigan X Baybay hybrid were studied. The effect of duration of exposure to liquid pre-culture media supplemented with varying levels of 2,4-D was also evaluated. Among the inflorescences tested, inflorescence no. 4 exhibited least browning and the highest incidence of calloid formation when pre-cultured in 2,4-D free liquid medium. Pre-culture duration of up to 3 days significantly reduced browning of the explants
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