Development of pressure-retaining type sampler for deep sea microorganisms
1991
Yamada, T. (National Research Inst. of Fisheries Engineering, Hasaki, Ibaraki (Japan)) | Hasegawa, K. | Ohwada, K.
Various microorganisms inhabit at deep sea in spite of the severe circumstances such as high hydraulic pressure, low temperature and darkness. In order to study the deep sea microorganisms bionomics, high performance sampler would be necessary for collecting microorganisms and keeping the in site circumstances. The pressure-retaining type sampler was developed for deep sea microorganisms to the depth of 7,000m. The functional aspects of the deep sea sampler comprise the following: -to preserve the in site conditions, such as hydraulic pressure and temperature, -to collect the deep sea water to enrich microorganisms by filtration method without contamination, -to compose the sampler simplify and for easy handling. The sampling vessel was designed in keeping the pressure within 95% of the in site value by means of a pressure preservative device with gas accumulator. The sampling vessel was made of titanium alloy to minimize the weight so that the disjointed vessel would have high handling performance. Also, slide type valve with O-ring seals was used in the sampling vessel so that the leak tight reliability has been improved. The result of the field test to the depth of 5,600m confirmed the possibility to preserve the pressure above 95% compared with the in site pressure
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