Chromosomal location of genes coding wheat gliadin proteins by high performance capillary electrophoresis analysis
1997
Yan, Y. (China Agricultural University, Beijing (China). College of Plant Science and Technology) | Prodanovic, S. | Surlan-Momirovic, G. | Zoric, D. | Liu, G.
Gliadin proteins of the hexaploid wheat variety "Chinese Spring" (CS) and its nullisomic-tetrasomic (NT) and ditelocentric (DT) aneuploid lines, were fractionated by a new, more powerful technique - High Performance Capillary Electrophoresis (HPCE). According to the presence, absence or amounts of gliadin peaks in NT and DT lines of the Chinese Spring variety, all were found to be controlled by genes on the short arms of group 1 and group 6 chromosomes (Gli-1 and Gli-2 loci). Of 45 components investigated, 27 were coded by the Gli-1 locus (5 by Gli-A1, 10 by Gli-B1 and 12 by Gli-D1), and 18 were coded by the Gli-2 locus (7 by Gli-A2, 7 by Gli-B2 and 6 by Gli-D2). Peaks 6 and 9 seemed to contain polypeptides controlled by loci on both 6A and 6B or 6B and 6D, respectively. The results support HPCE as a powerful tool for qualitative and quantitative genetic studies of wheat storage proteins.
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