Restriction fragment pattern (RFP) analysis of Aujeszky disease virus strains in Venezuela
1994
Morella, R. | Ivonne, C. (Instituto de Investigaciones Veterinarias Fonaiap (Venezuela))
Purified DNA 8 isolates of Suid herpesvirus I (SHV-I) collected during 1984-1992 from clinical outbreaks of Aujeszky disease on Venezuelan pig farms was compared by restriction fragment pattern (RFP) analysis, using PVU II and polyacrylamide gel electrophoresis (PAGE). The only difference detected between the strains 1984 and 1992 H was present in one fragment. The rest of the isolates analized presented differences in at least three fragments, that might be associated with loss and/or gain restriction endonuclease cleavage sites. Other two isolates collected from the same outbreak with one month of interval presented differences that might be associated with the passages in pigs as previously reported.
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