Detection of verocytotoxin-producing Escherichia coli from cattle feces by PCR using pooled samples
1997
Yokokoji, K. (Kagoshima-ken. Government Office (Japan)) | Fujimoto, H. | Uchizono, S. | Chuma, T. | Okamoto, K.
From May to October 1995 in Kagoshima Prefecture, the rectal contents of 100 slaughtered healthy cattle were inoculated onto the SIB agar, and pooled samples of IPA-negative colonies produced were surveyed by PCR for verocytotoxin genes. The genes were readily detected from 12 of rectal content, and the detection of verocytotoxin-producing Escherichia coli was efficiently performed with the following serological and biochemical examination
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