A two-dimensional electrophoretic analysis of serum proteins of rainbow trout (Oncorhynchus mykiss) treated with inflammation inducers
1998
Murata, M. (National Inst. of Industrial Health, Tokyo (Japan)) | Sugimoto, C. | Kodama, H. | Onuma, M.
Changes in serum proteins of rainbow trout (Oncorhynchus mykiss) treated with inflammation inducers or exposed to high temperature were analyzed by using two-dimensional gel electrophoresis employing isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fishes were inoculated with lipopolysaccharide (LPS) or Freund's complete adjuvant (FCA) as inflammation inducer, or exposed to water temperature shift from 12 to 25 degrees C. Increase of a protein of which apparent molecular weight of 43,000 and isoelectric point of 6.8 to 7.0 (43 kDa protein) was found in serum of fish inoculated with LPS or FCA. Contrary to this, few changes serum protein compositions were observed in fish exposed to high temperature. The results suggest that the 43 kDa protein induced by LOPS or FCA is a possible acute phase protein of rainbow trout
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