Enterobacterial repetitive intragenic consensus (ERIC) genotyping of Escherichia coli O 157:H7
2000
Ling, O.W. (Putra Univ., Selangor (Malaysia). Dept. of Biotechnology) | Radu, S. | Rusul, G. | Karim, M.I.A. | Purwati, E. | Lihan, S.
A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
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