The use of molecular genetic techniques to potato variety identification
2001
Polzerova, H. (Vyzkumny Ustav Bramborarsky, Havlickuv Brod (Czech Republic))
The RAPD procedure and SSRs analysis were used to study genetic diversity of 30 potato (Solanum tuberosum) genotypes, representing mainly Czech potato cultivars. Amplification with six decamer RAPD primers generated 66 DNA fragments, ranging in size from 178 bp to 1847 bp, of which 46 were polymorphic products. Twenty-two primer pairs flanking simple sequence repeats (SSRs) were examined for their potential use in DNA fingerprinting of potato. Total of 76 bands of sizes ranging from 98 to 500 bp were amplified, 56 bands were polymorphic. Similar matrixs derived from fourteen SSRs and six RAPD were able to distinguish selected cultivars. The utilisation of both approaches is discussed.
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