In vitro morphogenesis from seeds of Helianthus annuus L.
1999
Muhammed, A. | Sajid, M. | Hussain, I. | Quraishi, A. (National Agricultural Research Centre, Islamabad (Pakistan). Agriculture Biotechnology Inst.)
The objective of this study was to observe the in vitro morphogenetic response of a commercial hybrid SF-187 which is used in sunflower breeding programme. Basal MS media supplemented with NAA and BA was used. Cultures were incubated in complete obscurity at a temperature of 25 degree centigrade. Morphogenesis and ca4logenesis were observed after 14 days of incubation. When exposed to light, shootlets turned green within two days. A maximum of fifty plants were regenerated from single explants when cultured on MS medium supplemented with 2.0 mg/I each of BAP and NAA. The plantlets were rooted when subcultured on MS media supplemented with 0.5 mg/I IAA.
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