Analysis of volatiles emitted by some indica and new plant type varieties of rice (Oryza sativa L.) at vegetative and reproductive stages by solid phase microextraction and gas chromatography - mass spectrometry
2002
Zeng-Rong Zhu (International Rice Research Inst., Los Banos, Laguna (Philippines). Entomology and Plant Pathology Div.) Jimenez, R.R. | Cohen, M.B.
Previous field studies carried out the IRRI Experimental Station showed new plant type (NPT) prototype entries had significantly higher density of striped stem borer (SSB) egg masses during the rice growth season and higher density of SSB larvae and pupae during harvest than the improved indica dwarf varieties (IR64 and IR72). It was inferred that there might be some particular volatiles in the NPT entries which were attractants for SSB oviposition. Hence, a preliminary study was done to investigate the profile of volatile compounds released by the NPT rice entries, as well as by the indica varieties (IR64 and IR72), during the vegetative and reproductive stages. A technique found suitable for the fast and solventless extractions of the plant volatile was the solid phase micro-extraction (SPME) method. Subsequent qualitative screening and identification of extracted compounds was done by gas chromotography coupled with mass spectrometry (GCMS). The resulting total ion chromatograms (TIC) revealed NPT prototype rice lines (IR65564-, IR68011-) have significantly higher number of volatile compounds with higher peak intensities than the improved indica cultivars (IR64 and IR72). Further, there were fewer peaks in TIC indica varieties at reproductive than vegetative stage. Sesquiterpenes identified as zingibirene and other related isomers were found to be the major volatile compounds in the NPT prototype lines Hydrocarbons, heptadecane, pentadecane and a ketone, 2-tridecanone, were also detected
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