ESR spectroscopic characterization of free radicals: peroxyl radicals scavenging activity of sage during lipid oxidation
2001
Canadanovic-Brunet, J.M. | Djilas, S.M. | Cetkovic, G.S. | Nikacevic, I.R. (University of Novi Sad, Novi Sad (Yugoslavia). Faculty of Technology)
The formation of free radicals in a sample of sunflower oil during 600 minutes of catalytic oxidation was investigated by electron spin resonance (ESR) spectroscopy. In order to distinguish very reactive carbon- and oxygen-centered radicals, spin trapping technique, using reagent N-t-butyl-alfa-phenylnitrone (PBN), was applied. The results of the investigation indicated that in the first 150 min of reaction two different radicals, alkyl and alkoxyl radicals, were obtained. After this time alkyl, alkoxyl and peroxyl radicals were detected. The peroxyl radical scavenging activity of methanolic extract of sage (Salvia officinalis L.) was investigated in the described lipid system. Based on the obtained results it can be concluded that sage is a potential source of natural antioxidant which exhibits good peroxyl radical scavenging activity during sunflower oil oxidation.
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