Studies on the biosynthesis of enzyme xylanase by submerged fermentation from Aspergillus niger GCBMX-45
2002
Ikram-ul-Haq | Ehsan, A. | Butt, W.A. | Ali, S. (Government Coll., Lahore (Pakistan). Dept. of Botany)
The optimization of cultural condition was studied for the production of xylanase by mutant strain of Aspergillus niger GCBMX-45. Submerged fermentation was carried out in 250 ml Erlenmeyer flasks. Medium containing (% w/v) NaNO3, 0.1; Tween-80, 0.2; NH4Cl, 0.1; KH2PO4, 0.1; MgSO4.7H2O, 0.03; CaCl2.2H2O, 0.1; wheat bran, 2 was used for xylanase production. The enzyme production was maximum (195 U ml-1) at pH 4.5. One percent meat extract was added in medium as an additional organic nitrogen source.
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