A phenylalanine ammonia-lyase gene from asparagus: cDNA cloning, sequence and expression in response to wounding
2003
Bhowmik, P.K. (Kagawa Univ., Kagawa (Japan). Faculty of Agriculture) | Matsui, T. | Suzuki, H. | Kosugi, Y.
An asparagus (Asparagus officinalis L. cv. Welcome) cDNA clone coding the enzyme phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library prepared from asparagus spears using reverse transcription-polymerase chain reaction (RT-PCR). The partial cDNA clone encodes an mRNA of 527 by and the derived amino acid sequence is highly homologous to PAL from rice, maize and barley. Northern blot analysis showed increase level of pAS-ACO mRNA until 24 hour at 20 degree centigrade, which coincided well with PAL activity and fiber development, suggesting that the increase might be a response to the wounding associated with harvest.
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