HPLC Analysis and Extraction Methods of Decursin and Decursinlo Angelate in Angelica gigas Roots
2003
Kang, Y.G. | Lee, J.H. | Chae, H.J. (Hoseo University, Cheonan, Republic of Korea) | Kim, D.H. (Dankook University, Cheonan, Republic of Korea) | Lee, S.H. (Seoul National University, Seoul, Republic of Korea) | Park, S.Y. (Korea Food & Drug Administration, Seoul, Republic of Korea)
This paper is intended as an investigation of the analysis of high-performance liquid chromatoguaphy and the method of extraction of decursin and decursinol angelate in Angelica gigas roots. There are three kinds of extraction methods: distilled water, 50% EtOH and 100% EtOH. The condition of HPLC was obtained on a reversed-phase. column (Polarity dC, 4.6¡¿250mm, 5§) using a phosphate buffer-acetonitrile-sodium laury1 sulfate as the mobile phase. Under these chromatographic conditions, UV detector was 230mm, column temperature 30¡É and the speed of a current 1.0ml/min, respectively. The results of extraction with distilled water, 50% EtOH and 100% EtOH in Angelica gigas roots were as follows. the concentrations of decursin and decursinol angelate were 182 and 153 ppm (distilled water), 3,142 and 2,547 ppm (50% EtOH) and 3,341 and 2,778 ppm (100% EtOH). There were high positive correlations between the concentrations of decursin and EtOH (r=0.8928, p0.01) and decursinol angelate and EtOH (r
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