A histological analysis of regeneration in watermelon
2003
Ipek, M. | Kacan, H. | Sari, N. (University of Cukurova, Adana (Turkey). Department of Horticulture) | Curuk, S. (University of Mustafa Kemal, Antakya (Turkey). Department of Horticulture) | Cetiner, S. (University of Sabanci, Istanbul (Turkey). Faculty of Engineering and Natural Sciences) | Gaba, V. (ARO Volcani Center, Bet Dagan (Israel). Department of Virology)
The optimization of regeneration protocol for different genotypes increases the yield in transformation studies. Cotyledon explants of watermelon [Citrullus lanatus (Thunb) Matsum and Nakai] cm crimson Sweet were cultured on MS medium containing combinations of benzyl adenine (BA) (0,5,10,20 æM)and indole-3-acetic acid (IAA) (0, 0.5, 5 æM). Maximum shoot growth and subsequent rooting from explants on regeneration medium were obtained form the media containing 10æM BA + 0.5 æM IAA and 20 æM BA (75 and 78%) by direct organogenesis, respectively. Histological analysis showed that cell division was observed in the epidermal and subepidermal layers. Protuberant structures were observed in tissues between 7 and 12 days in culture. Meristematic structures were observed after 12 days in culture which later developed into buds.
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