Protoplast development of Petalonia binghamiae (Phaeophyta, Scytosiphonaceae) protoplast culture of P. binghamiae
2001
Wakabayashi, T. (Shizuoka Univ. (Japan). Faculty of Agriculture) | Tuboi, T. | Hara, M. | Kuboi, T.
Protoplasts were isolated from gametophytic thalli of Petalonia binghamiae in February 1995 and January 1999, then were cultured at 15 deg C under 14 h light (40 micto mol/square m/s) or 10 h light (20 micro mol/square m/s). The rate of differentiated colonies was counted on the basis of protoplasts in the February experiment. The epidermal protoplasts differentiated into a spherical cell chain and a protonemal filament. The rates of the two types were not affect by two different lighting conditions, and were 15 - 18% (fifth day) and 0.3 - 0.8% (tenth day), respectively. The cortical protoplasts were isolated at only January thallus (2.1% per total protoplasts), and a part of them grew into a spherical cell chain. The gametophytic thalli were regenerated from protonemal filaments in all culture (0.1%).
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