Antibacterial activity of ionized silver to four bacterial pathogens of freshwater fish
2001
Nakatsugawa, T. (Kyoto-fu. Inst. of Oceanic and Fishery Science, Miyazu (Japan)) | Yamada, S.
Four bacterial diseases, furunculosis,streptococcicosis, cold-water disease and bacterial hemorrhagic ascites mainly caused mass mortalities among pond-cultured freshwater fish. The antibacterial activity of ionized silver water (Ag water)to these bacterial pathogens was investigated by using silver plating fiber cotton (X-static, Sauquoit). Ag water were prepared as follows; 1. 5 g of silver cotton were immersed in 1l of sterile PBS (-) for 5 days. 0.22 micro m filtered PBS (-) were contained 0.94 mg/l of ionized silver. 2. 7 g of cotton were immersed in 500 rnlof PBS (-) for 7 days. 0.45 micro m filtered PBS (-) were contained 0.41 mg/l of ionized silver. Cultured four bacteria, Aeromonas salmonicida (As), Streptococcus iniae (Si), Flavobacterium psyclirophilum (Fp) and Pseudomonasplecoglossicida (Pp) were suspended with sterile PBS (-). 0.1 ml of suspended PBS was added into 10 ml of Ag water and cultured at 18 deg C. Viable cell counts were made at 4 and 24 hours after exposure. As (in Ag water 1), Si (in Ag water 1) and Fp (in Ag water 2) were counted < 10**2 CFU/ml at 4 hours. Pp (in Ag water 2) was < 10**1 CFU/ml at 24 hours. These results indicated ionized silver possessed the antibacterial activity to four bacterial pathogens.
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