Developmental competence of bovine embryos reconstructed by the transfer of somatic cells derived from frozen tissues
2001
Fahrudin, M. (Yamaguchi Univ. (Japan)) | Otoi, T. | Suzuki, T.
The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -35degC or -80degC in the presence of 5% DMSO were used as tissue explants to generate somatic cells. The resultant con fluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstructed from the somatic cells, 78% to 81% showed cleavage, 43% to 48% reached the stage of morula formation and 34% to 35% reached blastocyst formation. There were no significant differences in development (P>0.05) when the NT embryos were compared with those reconstructed from fresh somatic-cell-derived skin tissues (75%, 45%, and 38%, for cleavage, and development to morula and blastocyst stages, respectively). The results indicated that cell lines derived from bovine fetal skin cryopreserved by a simple method could be used as donors in nuclear transfer. The resulting embryos showed similar development in vitro to those reconstructed from un frozen fetal-skin-derived somatic cells.
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