Characterization of the base collection strains preserved in the Marine Microorganism Division, a sub-bank of the Microbial Culture Collections, Fish Research Agency
2004
Uchida, M. (National Research Inst. of Fisheries and Environment of Inland Sea, Ohno, Hiroshima (Japan)) | Sato, Y. | Murata, M. | Matsushima, R.
Abstract The Applied Microbiology Section in the National Research Institute of Fisheries Science is nominated as a Microbial Culture Collections Sub-Bank of the Gene Bank in the Fisheries Research Agency (FRA) and keeps a collection of fifty strains of bacteria and yeast as of May 2002, that are in preparation to be utilized as open cultures. Microbiological characterization was carried out with these collections to assist future culture utilization. The study was conducted using several commercial kits including NF-18 test (for gram negative microorganisms), NF-20 test (for gram positive microorganisms), API50CH test, and API zym20 test in addition to conventional phenotypic characterization. The partial nucleotide sequences of 16S rRNA gene (for bacteria) and 18S rRNA gene (for yeast) were also determined and submitted to DDBJ. Based on all the obtained results, tentative identification was conducted for the current fifty FRA collections.
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