Development of Doxorubicin overproducing Streptomyces Strain using Protoplast Regeneration
2003
Park, H.S. | Park, H.J. | Kim, Y.H. | Lim, S.M. | Kim, D.I. | Kim, E.S. (Inha University, Incheon, Republic of Korea) | Ryu, W.S. | Kim, S.L. (Boryung Pharmaceutical CO., Ansan, Republic of Korea)
Doxorubicin is an anthracycline-family polyketide compound with a very potent anti-cancer activity, typically produced by Streptomyces peucetius. In order to increase doxurubicin productivity, a semi-industrial doxorubicin-producing Streptomyces strain named BR-Dox was cultured in a R2YE liquid medium containing CaCO3, and then converted to a cell wall-free protoplast using lysozyme treatment method, followed by PEG-mediated cell wall regeneration.
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