Production and immobilization of an inulin fructotransferase (DFA I-producing) from Arthrobacter ureafaciens A51-1
2005
Haraguchi, K.(National Food Research Inst, Tsukuba, Ibaraki (Japan)) | Ohtsubo, K.
A bacterium strain A51 -1 producing an inulin fructotransferase (DFA I -producing) [EC 2.4.1.200] in the culture supernatant was isolated. The strain A51-1 was identified as Arthrobacter ureafaciens. The maximum enzyme activity (1.1 U/ml) was obtained with a medium containing 1.5% yeast extract. The enzyme showed maximum activity at pH 5.5 and 40 deg C. The enzyme was tolerant up to 70 deg C. An immobilized enzyme of an inulin fructotransferase (DFA I -producing) was prepared using Chitopearl BCW3510 as a carrier, for the first time. The immobilized enzyme can be used eight times without a significant loss of the activity.
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