Residue of oxytetracycline hydrochloride and alkyl trimethyl ammonium calcium oxytetracycline in the muscle of Perciform fish species
2006
Watanabe, K.(National Center for Stock Enhancement, Ohtsuki, Kochi (Japan). Komame Station) | Shima, Y. | Ashidate, M. | Nishioka, T. | Satoh, J. | Hotta, T. | Iida, T.
In this study, the twelve fish species employed were the red seabream Pagrus major, the black sea bream Acanthopagrus schlegelii, the yellowtail Seriola quinqueradiata, the purplish amberjack S. dumerili, the gold striped amberjack S. lalandi, the striped jack Psuedocaranx dentex, the long tooth grouper Epinephelus brunenus, the seven band grouper E. septemfaciatus, the red spotted grouper E. akaara, the three line grunt Parapristipoma trilineatum, the spotted parrot fish Oplegnathus punctatus, and girella Girella punctata. These fishes were fed moist pellets or dry pellets containing 50 mg/kg (BW) of oxytetracycline hydrochloride (OTC) or alkyl trimethyl ammonium calcium oxytetracycline (OTC-Q) for seven days (prescription period). Thereafter, feeds containing no antibiotics were provided to these fishes continuously for 20 days (OTC-Q fish) and 30 days (OTC fish) (resting period). The muscles of these fishes were individually sampled and stored at -80 deg C until analysis. Sampling was conducted the following day of the prescription and resting periods. Residue in these specimens as OTC was analyzed using High Performance Liquid Chromatography. Specimens which were sampled after prescription showed high concentrations of OTC. However, rested specimens showed values lower than those set under food safety standards for human consumption.
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