In vitro Examination of Chondroitin Sulfates Extracted Midduck (Styela clava) and Munggae Tunics (Halocynthia roretzi) as a Cosmetic Material
Kim, Y.R.;Shin, G.W.;Jung, T.S.(Gyeongsang National University, Jinju, Republic of Korea)E-mail:jungts@nongae.gsnu.ac.kr | Gyeongsang National University, Jinju, Republic of Korea(Division of Marine Bioscience/Institute of Marine Industry, Gyeongsang National University, Tongyeong, Republic of Korea) | Oh, M.J.(Yosu National University, Yosu, Republic of Korea)
With the aim of using a cosmetic material, chondroitin sulfates extracted from midduck tunics (Styela clava) and munggae tunics (Halocynthia roretzi) were examined in vitro with two cell lines for cell toxicity, collagen synthesis, cell growth and recovery ability after U.V. irradiation. Cell toxicity test with A 431 and CCD 1108Sk was able to observe high activity between 400 and 600 ㎍/m while standard chondroitin sulfate (CS) purchased from Sigma was showed at 80 ㎍/mL. Even fraction 1 and 2 collected from chondroitin sulfates originated from midduck appeared having the highest activity between 600 and 1000 ㎍/mL, but slightly lower compared to crude chondroitin sulfates from both mideduck and munggae. In cell growth examination, it was not able to find significant differences between chondroitin sulfates used.
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