Analysis of gamma-ray-induced DNA damage in human, mouse and rat peripheral blood lymphocytes using single-cell gel electrophoresis
Oh, H.;Jung, U.H.;Park, H.R.;Jo, S.K.(Korea Atomic Energy Research Institute, Daejeon, Republic of Korea)E-mail:skjo@kaeri.re.kr | Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)
The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of ∨60Co γ-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p less than 0.01) and TL (p less than 0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs.
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