The survey of Trichinella spiralis infection in finishing pigs using the pepsin-digestion method and ELISA in Korea
Seo, H.S.(Pfizer Animal Health Korea Ltd., Seoul, Republic of Korea) | Woo, G.H.(The University of Tokyo, Tokyo, Japan) | Youn, H.J.(Seoul National University, Seoul, Republic of Korea)E-mail:younhj@snu.ac.kr
Trichinella spiralis is one of the important zoonotic parasites with a wide variety of vertebrates hosts in nature. The purpose of this study were to analyze ESP(Excretory-Secretory Protein) antigen, to evaluate ELISA for the serological diagnosis of Trichinosis, and to survey T. spiralis infection in finishing pigs using the pepsin digestion method and ELISA in Korea. In the analysis of ESP antigen by SDS-PAGE and Western blot, 4 major bands (70, 55, 52.6, and 49 kDa) were revealed from the ESP antigen. Predilection sites of T. spiralis were the diaphragm, the tongue, masseter muscles, intercostal muscle, and hindlimb in orders in the experimentally infected rats.
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