Production of monoclonal antibody to 45 kDa somatic protein of Trichuris suis
Lee, J.K.(J.E.S., Seoul, Republic of Korea) | Kim, J.T.(Evergreen Animal Hospital, Seoul, Republic of Korea) | Seo, H.S.(Pfizer Animal Health Korea Ltd., Seoul, Republic of Korea) | Park, J.Y.(Bayer Korea Ltd., Seoul, Republic of Korea) | Youn, H.J.(Seoul National University, Seoul, Republic of Korea)
Trichuris suis does not excrete eggs during larval stage as well as in particular adult stage. It is impossible to diagnose by use of fecal examination method in those periods. Therefore, serological diagnostic method can be very useful for those stages. In order to produce monoclonal antibody, specific somatic and secretory-excretory (SE) antigens of T. suis were identified and analyzed by SDS-PAGE and Western blot. Monoclonal antibody-producing hybridoma cells were cloned, which were made of popliteal lymph node of BALB/c mice immunized with a 45 kDa somatic antigen of T. suis.
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