Assessment of Nucleus-DNA Damage in Red Pepper Cells Treated with γ-Radiation through Comet Assay
An, J.H.;Jeong, J.H.;Kwon, S.T.(Andong National University, Andong, Republic of Korea)E-mail:[email protected] | Back, M.H.;Kim, J.S.(Korea Atomic Energy Research Institute, Daejeon, Republic of Korea)
We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to ∨60Co γ-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced γ-radiation.
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