Production of Tropane Alkaloids by Optimal Culture Conditions in Adventitious Root of Hyoscyamus niger L.
2005
Park, D.J. (Gyeongsang National University, Jinju, Republic of Korea) | Kang, H.S. (Korea Biochemical Co., Uiryeong, Republic of Korea) | Min, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, Y.D. (Gyeongsang National University, Jinju, Republic of Korea) | Kang, S.M. (Gyeongsang National University, Jinju, Republic of Korea) | Jung, H.N. (Gyeongsang National University, Jinju, Republic of Korea) | Choi, M.S. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: mschoi@nongae.gnsu.ac.kr
Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and root were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best in root growth.
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