Comparison of Colorimetric Methods for the Determination of Flavonoid in Propolis Extract Products
2005
Kim, E.J. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea), E-mail: [email protected] | Lee, H.J. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea) | Kim, H.J. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea) | Nam, H.S. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea) | Lee, M.K. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea) | Kim, H.Y. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea) | Lee, J.H. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea) | Kang, Y.S. (Korea Food and Drug Administration, Seoul, Republic of Korea) | Lee, J.O. (Korea Food and Drug Administration, Seoul, Republic of Korea) | Kim, H.Y. (Gyeongin Regional Korea Food and Drug Administration, Incheon, Republic of Korea)
Quantitative analysis of flavonoids in commercial propolis extract products were compared by three colorimetric methods; aluminum chloride method, dinitrophenylhydrazine method and aluminum nitrate method. Aluminum nitrate method in Korea Health Supplement Food Code was proved to be specific only for flavones and flavonols same as aluminum chloride method, while dinitrophenylhydrazine method was specific for flavanones and dihydroflavonols. Therefore, the sum of flavonoid contents determined by 2,4-Dinitrophenylhydrazine method and aluminum nitrate method may represent the real content of total flavonoids.
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