Transformation and Mutagenesis of the Nematode-trapping Fungus Monacrosporium sphaeroides by Restriction Enzyme-mediated Integration (REMI)
2005
Xu, Jin (Yunnan University, Kunming, P.R. China) | Mo, Ming-He (Yunnan University, Kunming, P.R. China), E-mail: minghemo@yahoo.com.cn | Zhou, Wei (Yunnan University, Kunming, P.R. China) | Huang, Xiao-Wei (Yunnan University, Kunming, P.R. China) | Zhang, Ke-Qin (Yunnan University, Kunming, P.R. China)
In this study, the nematode-trapping fungus, Monacrosporium sphaeroides, was transformed with a plasmid harboring the hygromycin B phosphotransferase gene, via restriction enzyme-mediated integration (REMI). Frequencies of up to 94 transformants ㎍-¹ per linearized plasmid DNA were obtained by optimizing the PEG concentration, as well as the category and quantity of the added restriction enzyme. 90% of the transformants were determined to be stable for drug resistance when 20 randomly selected transformants were tested.
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